L-DOPA (3,4-dihydroxyphenyl-L-alanine) is a drug of choice in Parkinson's disease treatment. However the chemical method its synthesis has number drawbacks, so biotechnological approaches are being explored as an alternative. Aim. The goal to develop new affordable and effective biosynthesis using mushroom tyrosinase, immobilized economical carrier, which ensures stability enzyme multiple uses....

Replicative senescence occurs in normal cells, in contrast to their malignant counterparts which are generally immortal in vitro. We now show that induction of melanogenesis in subconfluent B16 melanoma cells deprived of growth factors can lead to irreversible growth arrest but continued cell viability, concurrent with the expression of specific glycosylated high molecular weight tyrosinases. T...

Tyrosinase enzymes (Tys) are involved in the key steps of melanin (protective pigments) biosynthesis and molecules targeting binuclear copper active site on tyrosinases represent a relevant strategy to regulate enzyme activities. In this work, possible synergic effect generated by combination known inhibitors is studied. For this, derivatives containing kojic acid (KA) 2-hydroxypyridine-N-oxide...

We have undertaken the purification of mushroom tyrosinase to obtain homogeneous enzyme for a study of its mechanism, which is unknown. Purified mushroom tyrosinases have already been reported (l-9). Some of these preparations were homogeneous, or almost homogeneous, by either electrophoresis or ultracentrifugation; one was homogeneous by both criteria (3, 9). However, there are discrepancies a...

the enzyme in M. leprae from the bacterial particles, by detergent-treatment, as a pure protein. The properties of 9-diphenoloxidase in the leprosy bacilli have been investigated and found to be different from tyrosinases occurring in mammalian and plant tissues. The enzyme has so far not been detected in any other mycobacteria, obtained from infected tissues of three different species of anima...

Tyrosinases and catechol oxidases belong to the family of polyphenol oxidases (PPOs). Tyrosinases catalyze theo-hydroxylation and oxidation of phenolic compounds, whereas catechol oxidases were so far defined to lack the hydroxylation activity and catalyze solely the oxidation of o-diphenolic compounds. Aurone synthase from Coreopsis grandiflora (AUS1) is a specialized plant PPO involved in the...