A new method for the simultaneous separation and determination of mercury(II) with other heavy and transition metals by ion chromatography (IC) was developed. This method involves on-column derivatization with 2,6-pyridinedicarboxylic acid (PDCA) and anion-exchange separation, followed by a post-column reaction with 2-[(5-bromo-2-pyridyl)-azo]-5-diethylaminophenol (5-Br-PADAP) to form metal–5-B...

UNLABELLED The polymerase basic 2 (PB2) subunit of the RNA polymerase complex of seasonal human influenza A viruses has been shown to localize to the mitochondria. Various roles, including the regulation of apoptosis and innate immune responses to viral infection, have been proposed for mitochondrial PB2. In particular, PB2 has been shown to inhibit interferon expression by associating with the...

The adsorption technique using chemically treated powder of bark of Acacia arabica was applied for the removal of toxic heavy metals from polluted waste water. The extent of adsorption found to be dependent on the pH and the time of contact. The adsorbent is effective for the quantitative removal of various toxic metals from the contaminated aqueous sample. The method is quite feasible, economi...

Post-translational modifications of viral proteins play important roles in regulating viral replication. Here we demonstrated that the PB2 of influenza A virus (IAV) can be modified by NEDD8. We revealed that E3 ligase HDM2 can promote PB2 NEDDylation. Overexpression of either NEDD8 or HDM2 can inhibit IAV replication, while knockdown of HDM2 has the opposite effect. Then we identified residue ...

Waste building sludge (WBS) originating in the production of concrete prestressed poles (CSW) and technical stone (TSW) used original Fe-modified forms (CSWFe, TSWFe) was tested as an environmentally friendly cheap sorbent selected cations (Cd2+, Pb2+, Cs+) anions (AsO43?, PO43?, CrO42?) from water. The experiments were performed with 0.1 0.5 mmol·L?1 model solutions a batch manner at laborator...

Proper management of waste produced by human activity has been one of the major environmental concerns of modern societies. The use of the waste as a source of renewable energy is an interesting option, increasing the country's energy matrix, reducing the volume of waste and mitigating its toxicity. However, for a proper design and operation of a waste-to-energy plant, it is essential to know t...

The RNA polymerase of influenza A virus is a host range determinant and virulence factor. In particular, the PB2 subunit of the RNA polymerase has been implicated as a crucial factor that affects cell tropism as well as virulence in animal models. These findings suggest that host factors associating with the PB2 protein may play an important role during viral replication. In order to identify h...

Adsorption desorption processes of Pb at contaminated levels in two variable charge soils were investigated. The red soil (RAR) developed on the Arenaceous rock (clayey, mixed siliceous thermic typic Dystrochrept) adsorbed more Pb2+ than the red soil (REQ) derived from the Quaternary red earths (clayey, kaolinitic thermic plinthite Aquult). The maximum adsorption values (Xm) that were obtained ...

as axon guidance cues. Both plexin B1 and plexin B2 are expressed during mouse kidney development. Inbred plexin B2 knockout (PB2 KO) mice die prenatally with defects in olfactory bulb development, neuronal proliferation and neural tube closure (exencephaly). We report here that these PB2 KO mice have defects also in kidney morphogenesis. In PB2 / embryos the kidneys are smaller and the number ...

In this report, we present a new method for visual detection of Pb2+. Gold nanoparticles (Au-NPs) were synthesized in one step at room temperature, using gallic acid (GA) as reducer and stabilizer. Pb2+ is added during the gold nanoparticle formation. Analysis of Pb2+ is conducted by a dual strategy, namely, colorimetry and spectrometry. During Au-NPs synthesis, addition of Pb2+ would lead to f...