New bioactive proteins need to be screened from various microorganisms for the increasing need for industrial and pharmaceutical peptide, proteins, or enzymes. A novel polymerase chain reaction (PCR) method, restriction site-dependent PCR (RSD-PCR), was designed for rapid new genes cloning from genomic DNA. RSD-PCR strategy is based on these principles: (i) restriction sites disperse throughout...

early diagnosis of bacteremia and its complications is the most important part of care and management of the febrile patients. however, a majority of patients who appear to be clinically septic have negative blood culture. the use of polymerase chain reaction (pcr) techniques has allowed identifying the pathogenic organisms rapidly and accurately. the objective of this study was to investigate ...

this study was conducted to detect the common bacteria implicated in bovine mastitis in bulk tank milk by polymerase chain reaction (pcr). forty-four milk samples from bulk tank milk were obtained and submitted to our laboratory. to detect staphylococcus aureus, streptococcus agalactiae, streptococcus dysgalactiae, escherichia coli, streptococcus uberis, and streptococcus parauberis, two sets o...

The reported genome-walking methods still suffer from some deficiencies, such as cumbersome experimental steps, short target amplicon, or deep background. Here, a simple and practical fork PCR was proposed for genome-walking. employs primer set of three random oligomers to implement walking task. In primary PCR, the low-stringency amplification cycle mediates binding places on genome, producing...

We have designed a universal PCR capable of amplifying a portion of the 16S rRNA gene of eubacteria, including Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus agalactiae, Streptococcus pneumoniae, Enterococcus faecium, Enterococcus faecalis, Mycobacterium tuberculosis, Legionella pneumophila, Escherichia coli, Klebsiella pneumoniae, Serratia marcescens, ...