The organization of the 5S RNA cluster has been analyzed in four strains of Drosophila melanogaster by the Southern technique. In some of the strains the 5S RNA cluster appears to be interrupted by an unrelated sequence. In other strains a continuous cluster is present.

Heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) is associated with clinical treatment failure. However, the resistance mechanism of hVISA has not been fully clarified. In the present study, comparative proteomics analysis of two pairs of isogenic vancomycin-susceptible S. aureus (VSSA) and hVISA strains isolated from two patients identified five differentially expressed prot...

A total of 26 Mobiluncus strains (17 M. curtisii and 9 M. mulieris strains) were compared serologically by double immunodiffusion and immunoblotting against antisera prepared against representative isolates of each species. All strains from the same species were strongly reactive with homologous antisera but generally weakly reactive with antisera to the heterologous Mobiluncus spp. The antiser...

BACKGROUND & OBJECTIVES Pulsed-field gel electrophoresis (PFGE) has been used for characterisation of group B streptococci, non-typeable by serotyping. We wanted to compare PFGE with serotyping in order to see the how well the method discriminates between epidemiological unrelated strains. METHODS A total of 78 epidemiological unrelated invasive GBS strains were examined by PFGE using SmaI di...

We used a genomic fingerprinting protocol to characterize 59 Staphylococcus aureus strains and a single S. intermedius isolate, all of which were previously typed by 13 different methods (F. C. Tenover et al., J. Clin. Microbiol. 32:407-415, 1994). These 60 strains were divided into three groups of 20 strains each, with each group including internal controls. Two of the three groups (groups SB ...

Fifty-nine Staphylococcus aureus isolates and 1 isolate of Staphylococcus intermedius were typed by investigators at eight institutions by using either antibiograms, bacteriophage typing, biotyping, immunoblotting, insertion sequence typing with IS257/431, multilocus enzyme electrophoresis, restriction analysis of plasmid DNA, pulsed-field or field inversion gel electrophoresis, restriction ana...

The aim of the work is to identify strains streptomycetes in genomes which there are nucleotide sequences similar gene cluster determining synthesis landomycin A (lan-cluster) and establish level similarity their primary structures organizations. Methods. Information on lan-cluster Streptomyces cyanogenus S136 chromosomal DNAs S. S136, laculatispora NRRL B-24909, griseoluteus JCM 4765 annotatio...

The minimal inhibitory concentrations of penicillin against 96 strains of group B streptococci and of methicillin against 10 strains of Staphylococcus aureus were unrelated to the growth phase of test bacteria. However, the minimal bactericidal concentrations were significantly higher in the stationary phase than the logarithmic phase for both organisms (P less than 0.001 and less than 0.05, re...

Eight strains of Legionella pneumophila were used to optimize cleavage of DNA with BssHII, SalI, or SpeI and separation by pulsed-field electrophoresis. Isolates from a community outbreak involving a contaminated hot tub were genomically identical. Cleavage patterns were distinctly different for unrelated environmental and nosocomial strains from a single hospital.