High molecular weight glutenin subunits (HMW-GS) are among the major determinant on wheat bread-making quality. Rapid ultra-performance liquid chromatography (UPLC) methods with high resolution and reproducibility for HMW-GS separation were established in this work. A sample could be completely analyzed in less than 12 min by using the optimized UPLC conditions: gradually increasing elution gra...

This paper presents an overview of possible new concept for modern teaching tool, Universal Programmable Logic Controller (UPLC). UPLC with the PM module and robo-platform represents an excellent experimental basis in the study of robotics. The proposed solutions are based on two years work and research.

An overview of the critical steps for the non-targeted Ultra-High Performance Liquid Chromatography coupled with Quadrupole Time-of-Flight Mass Spectrometry (UPLC-Q-ToF-MS) analysis of wine chemistry is given, ranging from the study design, data preprocessing and statistical analyses, to markers identification. UPLC-Q-ToF-MS data was enhanced by the alignment of exact mass data from FTICR-MS, a...

the veterinary drugs residues are also important pollutants found in milk, since veterinary drugs are commonly used in cattle management. considering the role of milk in human nutrition and its wide consumption throughout the world, it is very important to ensure the milk quality. a quantitative structure–retention relationship (qsrr) was developed using the partial least square (pls), kernel p...

The availability of reliable biomarkers of brain injury secondary to birth asphyxia could substantially improve clinical grading, therapeutic intervention strategies, and prognosis. In this study, changes in the metabolome of retinal tissue caused by profound hypoxia in an established neonatal piglet model were investigated using an ultra performance liquid chromatography - quadrupole time of f...

The advent of Waters UltraPerformance LC® (UPLC®) technology has enabled chromatographic separations to be routinely performed on columns packed with sub-2 μm particles at high linear velocity. Such separations result in high resolution, excellent throughput, and high sensitivity chromatographic analyses.1-2 These analytical qualities ensure the separation of coeluting metabolites and minimize ...

BACKGROUND Coptis chinensis Franch is a traditional Chinese medical herb. OBJECTIVE In this article, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to rapidly, qualitatively, and comprehensively identify the components in Coptis chinensis Franch. MATERIALS AND METHODS Chromatographic separation was achieved on an Agilent Zorbax RRHD...

Background 6-[18F]Fluoro-L-3,4-dihydroxyphenylalanine (FDOPA) is a frequently used radiopharmaceutical for detecting neuroendocrine and brain tumors and for the differential diagnosis of Parkinson's disease. To meet the demand for FDOPA, a high-yield GMP-compliant production method is required. Therefore, this study aimed to improve the FDOPA production and quality control procedures to enable ...

A rapid, high-throughput method employing ultra-performance liquid chromatography with tandem quadrupole mass spectrometry (UPLC-MS/MS) was developed and optimized for simultaneous quantification and confirmation of 64 pesticide residues and their toxic metabolites in fruit extracts prepared by a buffered QuEChERS procedure. The total time required for UPLC-MS/MS analysis was 8 min plus 2 min f...

The present study describes the development of a comprehensive science and risk based UPLC method and subsequent validation for the simultaneous analysis of related substances of omeprazole and domperidone active pharmaceutical ingredient (API) using a quality by design approach. An efficient experimental design based on systemat ic scuti ng of all three components of the UPLC method (column te...