Our studies of the venom from the Levantine viper Vipera lebetina have demonstrated the existence of both coagulants and anticoagulants in the same venom. We showed that V. lebetina venom contains: (1) proteases that degrade fibrinogen, but not fibrin; (2) fibrinolytic enzyme (lebetase); (3) factor X activator (VLFXA); (4) factor V activator (VLFVA). Fibrinolytic enzyme and VLFXA are metallopro...

Background:Envenomation by Macrovipera lebetina (M. lebetina) is characterized by prominent local tissue damage, hemorrhage, abnormalities in the blood coagulation system, necrosis, and edema. However, the main cause of death after a bite by M. lebetina has been attributed to acute renal failure (ARF). It is unclear whether the venom components have a direct or indirect action in causing ARF. T...

A researcher, a 40 year-old male, was bitten on his finger accidentally by a 75 cm long, adult male blunt-nosed viper, Macrovipera lebetina lebetina (Linnaeus) that had been collected from northern Cyprus during laboratory work in the Zoology Section, Biology Department of the Ege University Faculty of Science. The clinical signs, physiological and biochemical changes soon after the venomous bi...

We report the cloning and sequence analysis of Cerastes vipera and Macrovipera lebetina transmediterranea cDNAs coding for short non-RGD (Arg-Gly-Asp) disintegrins and for dimeric disintegrin subunits. The mRNAs belong to the short-coding class, suggesting that these disintegrin mRNAs may be more widely distributed than previously thought. Our data also argue for a common ancestry of the mRNAs ...

background:envenomation by macrovipera lebetina (m. lebetina) is characterized by prominent local tissue damage, hemorrhage, abnormalities in the blood coagulation system, necrosis, and edema. however, the main cause of death after a bite by m. lebetina has been attributed to acute renal failure (arf). it is unclear whether the venom components have a direct or indirect action in causing arf. t...

The venoms from central Asian snakes (Echis carinatus, Echis multisquamatus, Vipera ursini, Vipera lebetina, Agkistrodon halys halys and Naja naja oxiana) contain several enzymes with amidolytic- and procoagulant activity. We have characterized the activities and the mol. wts of the venom enzymes that are able to convert a number of commercially available chromogenic substrates for activated co...

Aim. In this study we focused on the search of fibrinogen-targeted proteases in venom Vipera lebetina. Methods. Fractionation was performed using FPLC chromatographic system Acta Prime Q Sepharose. Analysis protein mixtures SDS-PAGE. Аction blood coagulation analyzed APTT assay [2]. Proteolytic action fibrinogen and identification components with fibrinolytic activity electrophoresis mixture so...

In this study, the venom extract of five viper species (Vipera xanthina, Vipera ammodytes, Vipera kaznakovi, Vipera wagneri and Vipera lebetina) collected from various regions of Turkey was subjected to polyacrylamide disc electrophoresis. No pressure was applied to the venom glands to provide venom extract. Important differences were detected among the electrophoretic patterns of venom protein...