Shigella species (spp.) are gram-negative bacteria that are responsible for shigellosis. Although it can be controlled via antibiotics, increasing number of antibiotic resistant isolates of Shigella have been reported. Therefore, other strategies such as production of specific vaccine against this organism could be a suitable therapeutic approach. Attenuated live vaccines produced by gene delet...

mycoplasma agalactia was cultured in pplo broth medium supplemented with sterilized normal horse serum in fermentor. cultivation process continued at 37 ºc under aerobic conditions and growth was terminated after 26 hours cultured period with 2.2×109 mycoplasma per ml. samples were taken at different stages of growth. culture was inactivated with 0.04 %v/v formaldehyde and the vaccine was adjuv...

shigella species (spp.) are gram-negative bacteria that are responsible for shigellosis. although it can be controlled via antibiotics, increasing number of antibiotic resistant isolates of shigella have been reported. therefore, other strategies such as production of specific vaccine against this organism could be a suitable therapeutic approach. attenuated live vaccines produced by gene delet...

Hepatitis B virus (HBV) is a serious global health problem. The development of a safe and effective vaccinewould help infection prevention. Previous hepatitis B vaccine production involved the isolation of the noninfectious particle from chronic HBV carriers. DNA recombinant technology has been used for vaccineproduction without having been contaminated with blood-born infectious ag...

Background: There is a growing interest in development of an effective adjuvant system for improving DNA vaccines. Recent findings have confirmed an important role for autophagy in both innate and adaptive immunity. The current study was undertaken to determine the efficacy of autophagy induction with Beclin-1, as a novel adjuvant system, in mice immunized with human papilloma virus (HPV) DNA v...

knowledge of virus and replication kinetic is one of the most important issues in the vaccine production. the present study aimed to evaluate the best harvesting time of h9n2 avian influenza virus (aiv) vaccine strain inoculated in specific pathogen free (spf) embryonated chicken eggs (ece)s. for this purpose, 10-5 dilution of aiv (a/chicken/iran/99/h9n2) was inoculated into 336, 11-day old ece...

  Blackleg in cattle has been recognized since 1938 in Iran that mostly affects cattle in enzootic farm. Main object of this study was to prepare and formulate a concentrated potent vaccine for immunization of cattle against blackleg in Iran. Experimental concentrated blackleg vaccine was prepared according to the method described by FAO. The medium (Modified medium for production of experiment...