A model was developed to study the multiplication of various Legionella spp. in tap water containing Hartmannella vermiformis. Tap water cultures prepared with the following components were suitable for the multiplication studies: Legionella spp., 10(3) CFU/ml; H. vermiformis, 10(4.4) cysts per ml; and killed Pseudomonas paucimobilis, 10(9) cells per ml. Cocultures were incubated at 37 degrees ...

The study of amoeba-associated Chlamydiae is a dynamic field in which new species are increasingly reported. In the present work, we characterized the developmental cycle and analyzed the genome of a new member of this group associated with Vermamoeba vermiformis, we propose to name "Rubidus massiliensis." This bacterium is well-adapted to its amoeba host and do not reside inside of inclusion v...

In this study, we report the isolation of a new giant virus found in sewage water from the southern area of Jeddah (Saudi Arabia), with morphological and genomic resemblance to Faustoviruses. This new giant virus, named Kaumoebavirus, was obtained from co-culture with Vermamoeba vermiformis, an amoeboid protozoa considered to be of special interest to human health and the environment. This new ...

Free-living amoebas (AVLs), such as Acanthamoeba, Naegleria, Balamuthia, and Vermamoeba, have been identified in both natural man-made settings, hot springs spas. Naegleria fowleri causes primary amebic meningoencephalitis, while Acanthamoeba Balamuthia cause chronic granulomatous encephalitis. can also skin lesions amoebic keratitis. This study aimed to identify the presence of these protozoa ...

Evidence of associations between free-living amoebas and human disease has been increasing in recent years. Knowledge about phylogenetic relationships that may be important for the understanding of pathogenicity in the genera involved is very limited at present. Consequently, we have begun to study these relationships and report here on the phylogeny of Hartmannella vermiformis, a free-living a...