The constant threat of newly emerging influenza viruses with pandemic potential requires the need for prompt vaccine production. Here, we utilized the Vero cell polymerase I (PolI) promoter, rather than the commonly used human PolI promoter, in an established reverse-genetics system to rescue viable influenza viruses in Vero cells, an approved cell line for human vaccine production. The Vero Po...

introduction: it is now generally accepted that vero cells provide beneficial effects on improving embryo quality in many animal species. the aim of this research was to determine the effect of vero cell co-culture on the development of vitrified 8-cell mouse embryos. material and methods: eight- cell mouse embryos were provided from superovulated female nmri mice and divided into treatment cas...

background: this study was undertaken to investigate the efficiency of two different embryo somatic cell co-culture conditions tissue culture medium 199 (tcm199)–vero cells and menezo b2 (b2)-vero cells for the in vitro developmental quantity and quality of bovine embryos. materials and methods: bovine oocytes were allowed to mature and subsequently undergo fertilization in vitro. their presump...

The Vero SBRT system was benchmarked in a planning study against the Novalis SRS system for quality of delivered dose distributions to intracranial lesions and assessing the Vero system's capacity for SRS. A total of 27 patients with one brain lesion treated on the Novalis system, with 3 mm leaf width MLC and C-arm gantry, were replanned for Vero, with a 5 mm leaf width MLC mounted on an O-ring...

OBJECTIVES This study aims to investigate the adhesion characteristics between submicron calcium oxalate dihydrate (COD) with a size of 150 ± 50 nm and African green monkey kidney epithelial cells (Vero cells) before and after damage, and to discuss the mechanism of kidney stone formation. METHODS Vero cells were oxidatively injured by hydrogen peroxide to establish a model of injured cells. ...

DTS-II is a highly diphtheria toxin (DT)-sensitive cell line previously isolated by transfection of wild-type DT-resistant mouse L-M(TK-) cells with the cDNA encoding a monkey Vero cell DT receptor. DTS-II cells are as toxin-sensitive as Vero cells, have approximately 3-fold more receptors than Vero cells, and have approximately 10-fold lower affinity for DT than Vero cells. We now cotransfecte...

Plaque formation in Vero cells has been induced with seven rabies serogroup viruses either cocultivated as infected BHK-21 or Aedes albopictus cells, or directly cultivated as infected mouse brain.

measles has been a major cause of illness and death in children and vaccination against the disease is part of the who global immunization program. a suitable vaccine should create maximum immune response against the pathogen and must be safe for the user. thus, after production, vaccines must be analyzed and controlled by the producer and confirm by relevant governmental organizations. the foo...

poliomyelitis, an acute viral infectious disease caused by poliovirus, still remains a public health problem in developing countries. despite the global effort to eradicate polio, continuing the polio immunization with a potent and safe vaccine is essential. for accurate vaccine evaluation, three types of cell lines including hela, hep2c and vero were evaluated and compared using two methods of...

This work investigated the replication kinetics of the four dengue virus serotypes (DEN-1 to DEN-4), including dengue virus type 4 (DEN-4) recovered from an infectious cDNA clone, in Vero cells and in MRC-5 cells grown on Cytodex 1 microcarriers. DEN-1 strain Hawaii, DEN-2 strain NGC, DEN-3 strain H-87, and DEN-4 strain H-241 , and DEN-4 strain 814669 derived from cloned DNA, were used to infec...