Very high gravity (VHG) fermentation is aimed to considerably increase both the fermentation rate and the ethanol concentration, thereby reducing capital costs and the risk of bacterial contamination. This process results in critical issues, such as adverse stress factors (ie., osmotic pressure and ethanol inhibition) and high concentrations of metabolic byproducts which are difficult to overco...

Fuel ethanol is an important renewable and sustainable fuel, produced in China by fermentation of mostly corn, wheat and cassava feedstock. Fermentation produces an ethanol-lean broth (10 to 12 vol%). Ethanol is recovered by distillation, followed by a molecular sieve drying beyond the azeotropic point. The distillation and molecular sieve operations consume most of the total energy used, with ...

BACKGROUND The optimization of industrial bioethanol production will depend on the rational design and manipulation of industrial strains to improve their robustness against the many stress factors affecting their performance during very high gravity (VHG) or lignocellulosic fermentations. In this study, a set of Saccharomyces cerevisiae genes found, through genome-wide screenings, to confer re...

BACKGROUND VHG fermentation is a promising process engineering strategy aiming at improving ethanol titer, and thus saving energy consumption for ethanol distillation and distillage treatment. However, sustained process oscillation was observed during continuous VHG ethanol fermentation, which significantly affected ethanol fermentation performance of the system. RESULTS Sustained process osc...

BACKGROUND Classical strain engineering methods often have limitations in altering multigenetic cellular phenotypes. Here we try to improve Saccharomyces cerevisiae ethanol tolerance and productivity by reprogramming its transcription profile through rewiring its key transcription component RNA polymerase II (RNAP II), which plays a central role in synthesizing mRNAs. This is the first report o...

The ethanol fermentation efficiency of sweet sorghum stem juice (SSJ) under a very high gravity (VHG) condition (250 g/L sugar) was improved by immobilized Saccharomyces cerevisiae SSJKKU01, using stirred tank bioreactor (STR) coupled with column (CR). Dried rattan pieces (as carriers for cell immobilization) at 50% the working volume CR were suitable use in batch fermentation. average concentr...

Understanding altered metabolism is an important issue because altered metabolism is often revealed as a cause or an effect in pathogenesis. It has also been shown to be an important factor in the manipulation of an organism's metabolism in metabolic engineering. Unfortunately, it is not yet possible to measure the concentration levels of all metabolites in the genome-wide scale of a metabolic ...

Over the last decades, constant growth of world-wide industry has been leading to more and concerns with its direct impact on greenhouse gas (GHG) emissions. Resulting from that, rising efforts have dedicated a global transition an oil-based cleaner biotechnological processes. A specific example refers production bioethanol substitute traditional transportation fuels. Bioethanol produced for de...

This study focused on the detection of value-added co-products in dried distiller's grain plus soluble (DDGS), a possibility that could open new avenues for further processing and marketing of DDGS and improving economic sustainability of ethanol industry. Varieties of triticale, wheat and two benchmarks, CPS wheat and Pioneer Hi-Bred corn, were fermented using two very high gravity (VHG) ferme...