Two experiments were conducted to study the effects of methionine supplementation on ruminal fermentation and digesta kinetics. In Exp. 1, nine ruminally cannulated beef heifers (average initial BW = 527 kg) in a crossover design were fed low-quality grass hay and cottonseed meal with or without 11.4 g of supplemental methionine (polysaccharide-coated). Particulate and fluid kinetics, rate of D...

Two experiments using six rumen-fistulated Holstein steers in 3 X 3 Latin square designs were conducted to test the effects of urea addition to corn silage, and grain addition to haylage, on feed intake, digestibility of nutrients and rumen fermentation of complete diets fed once daily. Addition of .34 and .68% urea to corn silage increased dietary crude protein from 8.1 to 11.3 and 13.3%, and ...

different levels of grape pomace extract (ge) were evaluated for their effect on in vitro gas production and as well on some such ruminal fermentation parameters as apparent degraded substrate, volatile fatty acids (vfa), and microbial protein production, in sheep, as ruminal inoculum donor. as for extraction, dried grape pomace and distilled water were mixed in an equal weight ratio. ge was ad...

Four multiparous, ruminally fistulated Angus x Hereford cows (average BW = 568 kg) were bred to the same bull and used to monitor periparturient changes in DMI, ruminal capacity, and digestion and fermentation characteristics. Cows were individually fed alfalfa hay (16.0% CP, 51.3% NDF) at 130% of the average DMI of the previous 5-d period. Ruminal capacity and DM fill were measured once every ...

The effect of Bacillus subtilis natto on hindgut fermentation and microbiota of early lactation Holstein dairy cows was investigated in this study. Thirty-six Holstein dairy cows in early lactation were randomly allocated to three groups: no B. subtilis natto as the control group, B. subtilis natto with 0.5×10(11) cfu as DMF1 group and B. subtilis natto with 1.0×10(11) cfu as DMF2 group. After ...

Th is experiment compared linear relationships among end-products of rumen fermentation measured at the time (t1⁄2) at which a feed produces half of its asymptotic gas production) or at 48 h. Meadow hay and corn grain were incubated for t1⁄2 (16 and 9 h, respectively) or for 48 h into glass bottles. Each bottle (310 ml) was fi lled with feed sample (0.5 g) and 75 ml of buff ered rumen fl uid, a...

To access the fermentative response of equine caecal microbial population to nitrogen availability, an in vitro study was conducted using caecal contents provided with adequate energy sources and nitrogen as limiting nutrient. Two nitrogen (N) sources were provided, protein (casein) and non-protein (urea). Caecal fluid, taken from three cannulated horses receiving a hay-concentrate diet, was mi...

The effect of phosphorus supplementation to ammoniated rice straw was studied. The in vitro experiment was carried out following the first stage of Tilley and Terry method. The treatments consisting of four diets were A= 50% ammoniated rice straw + 50% concentrate (control), B= A + 0.2% phosphorus (P) supplement, C= A + 0.4% phosphorus (P) supplement, and D= A + 0.6% phosphorus (P) supplement o...

This study aimed to evaluate the microbial rumen population, fermentability, and digestibility of Moringa leaf supplementation in dairy cow ration using vitro determine optimal level supplementation. The experiment consist two steps with first step was microbiology measurement used a Randomized Block Design 5 treatments extract (P0= control; P1= 5%, P2 = 10%, P3 =15%, P4 =20%) second fermentabi...

the effect of eucalyptus (eucalyptus globulus labill;egeo) and angelica (heracleum persicum desf. ex fischer; hpeo) essential oils was assessed at the levels of 0, 3, 30, 300 or 3000 (µl 30 ml-1) on in vitro fermentation of buffered rumen fluid from 3 castrated male sheep. the fermentation kinetics were estimated after 54 hours incubation. the fermentation kinetic values, in vitro gas productio...