We describe the polyphasic characterization of four Vibrio isolates which formed a tight AFLP group in a former study. The group was closely related to V. cyclitrophicus, V. lentus and V. splendidus (98.2-98.9% similarity) on the basis of the 16S rDNA sequence analysis, but by DNA-DNA hybridisation experiments it had at maximum 61% DNA similarity towards V. splendidus. Thus, we propose that the...

Oysters are permanently exposed to various microbes, and their defense system is continuously solicited to prevent accumulation of invading and pathogenic organisms. Therefore, impairment of the animal's defense system usually results in mass mortalities in cultured oyster stocks or increased bacterial loads in food products intended for human consumption. In the present study, experiments were...

The development of intensive aquaculture industry has aggravated the dangers ammonia and nitrite in aquatic ecosystems. Probiotics have been widely used to maintain improve water quality by removal aquaculture. In this study, two facultative anaerobic strains AB15 NB14, capable efficient removal, were isolated from biofloc, which acts as a natural micro-flora storeroom shrimp culture ponds, ide...

A total of 209 pathogenic vibrios strains were isolated from Muthupettai mangrove environment, Southeast coast of India. All strains were identified to be 8 species (Vibrio cholera, Vibrio harveyi, Vibrio mimicus, Vibrio splendidus, Vibrio aestuarianus, Vibrio vulnificus, Vibrio parahaemolyticus and Vibrio metschnikovii) from all stations. The levels of resistance of bacteria to various antibio...

Cleaner fish, i.e. various wrasse (Labridae) species and lumpsucker Cyclopterus lumpus, are to an increasing extent used for biocontrol of the salmon louse Lepeophtheirus salmonis in European salmon farming. Although efficient de-licers, cleaner fish mortality levels in salmon farms are often high. Bacterial infections are common, and Vibrio splendidus-related strains are frequently identified ...

16S rRNA molecules in a microbial strain can differ due to nucleotide variation between their genes. This is a typical trait of fast-growing bacteria to cope with different niches. We investigated characteristics of 16S rRNA genes in Vibrio splendidus strain PB1-10, from the normal flora of Atlantic halibut. Sequencing of 16S rRNA gene clones detected 35 variable positions in a total of 13 diff...

The Vibrio splendidus clade is the biggest in Vibrionales composed of 11 described species (25). Diversification of these species may have occurred 260 million years ago. The main driving forces of speciation in this clade have never been studied. Population biological parameters (population base recombination rate (ρ), population base mutation rate (θ), and index of association (Ia)) were dete...

Introduction It is well known that Vibrio species constitute an important percentage of the heterothrophic bacteria associated with marine bivalves, especially oysters, mussels and clams. Several Vibrio species can be pathogenic and are associated with disease outbreaks, therefore the accurate identification of these microorganisms is important. Vibrio spledidus has been found to be a dominant ...

We analyzed the usefulness of rpoA, recA, and pyrH gene sequences for the identification of vibrios. We sequenced fragments of these loci from a collection of 208 representative strains, including 192 well-documented Vibrionaceae strains and 16 presumptive Vibrio isolates associated with coral bleaching. In order to determine the intraspecies variation among the three loci, we included several ...

a total of 116 marine fishes and sediments were collected from persian gulf shore near bushehr in order to isolate vibrio species. after culturing on thiosulphate- cirate- bile salts- sucrose and with biochemical tests, 30 isolates showed vibrio characteristics which were categorized in 7 vibrio species, v. anguillarum, v. alginalyticus, v. harveyi, v. vulnfiicus, v. ordalii, v. carchariae and ...