نتایج جستجو برای: viral antigens
تعداد نتایج: 256276 فیلتر نتایج به سال:
introduction: hela cell is a widely studied cell line derived from human cerzix adenocarcinoma. the cells are epithelial - like in morphology and susceptible to polio viruses and adenovirus type 5. hela cells are usually as monolayer for isolation and propagation of some human viruses, as well as for the expression of recombinant proteins, including hepatitis b surface antigen. the aim of this ...
In this study One lymph node from cow with viral lymphosarcoma and one lymph node from healthy cow were analyzed by three different methods for protein extraction, include simple homogenizing in PBS, sonication and phenol extraction. Tumor antigen detection relies on immunoprecipitation followed by SDS-PAGE. In this experiment Streptococcus pyogenes was used for purification of immune compl...
the objective of this study was to determine the protein pattern and antigenic structure of bhv-1. ten viral isolates from cows with clinical signs of respiratory infections were selected for this study and confirmed by sn test. all samples were cultured on cell cultures and purified by ultracentrifugation. purified isolates were electerophorsed using sds-page method and immunoblotted. vi...
Several lines of evidence are presented to suggest that histocompatibility antigens can be physically associated on the cell surface with viral antigens and possibly other foreign antigens. The lysis of the murine tumor cells EL4 and P388 by syngeneic cytotoxic lymphocytes was inhibited by antisera directed against the H-2 antigens on the tumor cells, consistent with the hypothesis that H-2 ant...
DNA-fragments coding for a variety of different viral antigens have been cloned and expressed in E. coli. Selected purified recombinant antigens were used for detection of specific antibodies by the means of ELISA technique. This approach has been used for the development of four different ELISA's for the detection of HIV- and EBV-specific antibodies.
Kaposi sarcoma (KS) is a debilitating, disfiguring and often fatal disease. The tumor is always associated with Kaposi sarcoma herpesvirus (KSHV, also known as HHV8) [1]. It is often, perhaps always, associated with immune deficiency. In transplant recipients KS is associated with pharmacologic immunosuppression, while in HIV patients KS is associated with low CD4 T cell counts. Improvement in ...
Background and Aims: Several drugs are being used in treatment of HSV infection in human but still introducing an effective safe drug is desirable. Methods: We investigated the inhibitory effect of morphine on replication of HSV in vitro. Results: The results indicated that a concentration of up to 200 ug/ml morphine had a limited effect on Vero cell viability. At this concentration the growt...
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