Avian influenza H9N2 subtype viruses have had a great impact on Iranian industrial poultry production economy since introduction in the country. To approach Rapid and precise identification of this viruses as control measures in poultry industry, a real time probe base assay was developed to directly detect a specific influenza virus of H9N2 subtype -instead of general detection of Influenza A ...

Herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) are members of the Herpesviridae family. About 40% to 80% of the world populations are infected with HSV and its prevalence is high in Iran. The high prevalence of this virus in the community and the ability of the virus in causing fatal diseases among immunocompromised patients, have encouraged studies to be performed on HSV and suitable cell ...

bluetongue is an acute and subacute viral diseases of domestic and wild ruminants.this research was conducted to assess the disease status using serological diagnostic methods and virus isolation were performed. during the period 2003-2006, from sheep farms of 9 provinces including markazi, ardabil, yazd, qom, east azarbaijan, west azarbaijan, qazvin, isfahan and golestan, four handred thirty t...

Translation and replication of positive stranded RNA viruses are directly initiated in the cellular cytoplasm after uncoating of the viral genome. Accordingly, infectious virus can be generated by transfection of RNA genomes into susceptible cells. In the present study, efficiency of conventional virus isolation after inoculation of cells with infectious sample material was compared to virus re...

background influenza type a (h1n1) causes an epidemic disease, resulting in thousands of deaths throughout the world. objectives our aim was comparing the efficacy of two different methods in isolating the influenza (h1n1) virus including cell culture and real-time pcr in tabriz and suburbs. patients and methods of throat swab samples, 220 were collected in viral transport medium (vtm) from pat...

background: influenza a viruses bearing all known ha and na subtypes have been isolated from avian hosts: but only the a/h1n1,a/h2n2 and a/h3n2 influenza subtypes have been associated with widespread epidemics in humans un­til now .while influenza b detected only in humans. subtype h5 infection in humans occurred for the first time dur­ing 1997-1998 in hong kong, then china in 2003, vietnam and...

Isolation of Japanese encephalitis (JE) virus using C6/36 cell and immunofluorescence virus antigen detection techniques was attempted from female mosquitoes collected with CDC gravid traps in Samut Songkhram Province in the central region and in Phuket Province in southern Thailand, in 2003. One thousand and eighty female mosquitoes including 6 species of the Culicidae family (Culex quinquefas...

Taking the superiority of the connection of the network, computer viruses bring more harm to users. However few researches focus on the network isolation against virus. Most existing isolation tools trying to isolate the faults and protecting the specific sub-network do not fit to prevent web-spreading network virus. By dividing the network connection into subconnections the paper presents sub-...

isolation of bohv-1 was attempted from nasal swabs as well as aborted foetuses and vaginal swabs in madin darby bovine kidney (mdbk) cell line to find out the prevalent strain in the state of west bengal, india. the virus was isolated from only one case out of 65 nasal swabs whereas no virus was isolated from aborted foetuses and vaginal swabs. the isolate was typed as bohv-1.2 (strain india 3).