background & aim: considering that antioxidants are known as effective free radicals scavenger, it may be able to improve the in vitro oocyte maturation and the fetal quality. this study was designed to determine the effect of gundelia tournefortii leaves (gte) hydro-alcoholic extract as a source of antioxidant on in vitro oocyte maturation. experimental: germinal vasicle (gv) were recovere...

background & aim: oocytes in vitro maturation is an enormously promising technology for the treatment of infertility, yet its clinical application remains limited owing to poor success rates. therefore this study was designed to determine the effect of gundelia tournefortii root hydro alcoholic extract as a source of antioxidant on in vitro oocyte maturation. experimental: in this study, th...

background:   objective: in this study, we supplemented a chemically defined medium with different combinations of selenium, calcium and calcium ionophore concentrations to obtain the best rate of human oocytes maturation, survival, and fertilization.   materials and methods: as an experimental study, three combinations of [selenium (5 μg/ml), calcium (5 μg/ml) and calcium ionophore (1 μg/ml)],...

background: the aim of this study was to assess effects of cysteamine as an anti-oxidant on rate of in vitro maturation of oocyte and determination of its effects on spindle size and shape. methods: pre-mature mice were primed with pregnant mare stimulating gonadotrophin (pmsg) and germinal vesicle (gv) stage oocytes were obtained 48 h after. the oocytes were cultured in tissue culture medium (...

the use of ovarian stimulation for infertility treatment is associated with side effects of ovarian hyperstimulation syndrome (ohss) and potential cancer risk. this is also true in high risk women such as those polycystic with ovary (pco) and polycystic ovarian syndrome (pcos). in vitro maturation (ivm) of oocytes was primarily developed to make ivf safe for women with pco and at high risk of o...

background: vitrification has proven to be more effective than slow freezing methods to cryopreserve mammalian oocytes. the objectives of this study were to evaluate the effects of vitrification on immature and in vitro matured, denuded and cumulus compact goat oocytes and their subsequent fertilization. methods: oocytes were either cryopreserved as immature cumulus compact (imcc) (n=98 exp 1; ...

background: it is important to protect oocytes and embryos from oxidative stress in the culture medium. melatonin has been shown to be a direct free radical scavenger. objective: effect of melatonin during in vitro oocyte maturation, fertilization and embryo development of mouse oocytes was evaluated. materials and methods: oocytes from supper-ovulated mouse were divided to two groups: cumulus ...

Background: The maturation medium for in-vitro oocyte maturation is usually supplemented with serum. However, supplementation of serum from pregnant women adversely affects the outcome of in-vitro maturation. The purpose of the study was to assess if growth factors or granulosa cell coculture could overcome the adverse effects of pregnant women’s serum. Materials and Methods: The basal maturati...

Background: In vitro culture of ovarian follicles may preserve fertility in women with premature ovarian failure due to cancer .It seems that creation a condition that could maintain cellular communications and supports growth of follicles to produce mature oocytes appear to be essential. Nitric oxide (NO) has been recently shown to act with a dual action in mouse oocyte meiotic maturation depe...

Background: The pregnancy rates from oocytes matured in vitro are much lower than those of in vivo stimulation cycles, indicating that optimization of IVM remains a challenge. Reduced developmental competence of oocytes matured in vitro indicates that current culture systems for oocytes maturation do not adequately support nuclear and/or cytoplasmic maturation. Therefore in this study, we evalu...