We characterized 24 polymorphic tetranucleotide microsatellite loci for delta smelt (Hypomesus transpacificus) endemic to the San Francisco Bay Estuary, CA, USA. Screening of samples (n = 30) yielded two to 26 alleles per locus with observed levels of heterozygosity ranging from 0.17 to 1.0. Only one locus deviated from Hardy-Weinberg equilibrium, suggesting these individuals originate from a s...

Background & Aims: SLC26A4 gene mutations are the second identifiable genetic cause of autosomal recessive nonsyndromic hearing loss (ARNSHL) after GJB2 mutations and are currently investigated in molecular diagnosis.In databases, several potential STR markers related to this region have been introduced. In this investigation, the characteristics and informativeness of D7S2425 CA repeat STR mar...

mahi sefid (cyprinidae) is an economically important fish in the south caspian sea. specimens was collected from the lamir river, sefid rud, shir rud and tajan rivers and analyzed at 38 microsatellite dna loci. eight primers out of the thirty analyzed loci were polymorphic in all sampling regions. the highest observed heterozygosity (1) was in locus ca1 in sefid rud and lowest was 0.07 in locus...

association of genetic polymorphism in the calpastatin (cast) gene with average daily gain was examined in iranian purebred kurdi sheep. the genotypes for cast were determined by the pcr-sscp method. blood samples were collected from 84 purebred kurdi sheep belonging to the kurdi breeding station located in the khorasan province, north-east of iran. extraction of genomic dna was based on the gu...

Information on the genetic structure of marine species is essential for stock improvement programs. In orderto analyses the genetic diversity of the Hawksbill turtle (Eretmochelys imbricte) by the microsatellite geneticmethod, 64 samples were caught from the beaches located in Kish and Qeshm islands. Polymerase chainreactions (PCR) of genomic DNA extracted from the samples wer...

Genotypes for melatonin receptor type 1A (MTNR1A) and Calpastatin (CAST) were determined by enzymatic digestion of PCR products and Calpain(CAPN) genotype detected by PCR-SSCP method in Iranian Karakul sheep. Blood samples were collected from 100 purebred Karakul sheep. The extraction of genomic DNA was based on guanidinium thiocyanate- silica gel method. PCR amplicons were digested with restri...

the population genetic structure of the persian sturgeon (acipenser persicus) in the sefidrud and gorganrud river watersheds was analyzed based on the characterization of microsatellite markers. one hundred fin clip samples of persian sturgeon from the two regions were collected. four microsatellite loci (ls68, spl168, spl173, and afu68) were analyzed for molecular characterization, resulting i...

Hardy-Weinberg equilibrium (HWE) is the state of the genotypic frequency of two alleles of one autosomal gene locus after one discrete generation of random mating in an indefinitely large population: if the alleles are A and a with frequencies p and q(=1-p), then the equilibrium gene frequencies are simply p and q and the equilibrium genotypic frequencies for AA, Aa and aa are p2, 2pq and q2. I...

quantitative trait loci (qtl) affecting milk production traits on bovine chromosome 6 close to the opn gene location have been identified through several whole genome scans. opn is a phosphoprotein, synthesized in a variety of tissues and cells and secreted into body fluids. in this study, dna was isolated from blood samples collected from 398 holstein cows in tehran and esfahan provinces. dna ...