01W AND GRONWALL (1) [N 1952 first applied the histochemical method of Hotchkiss for staining carbohydrates. In principle the polysaccharides are oxidized by periodic acid to give polyaldehydes, which yield violet-red compounds with Schiff ‘s reagent, fuchsin sulfite. K#{246}iw’s procedure, although giving excellent results when the glycoprotein was separated electrophoretically on filter paper...

A passive dosimeter designed to measure exposure to hydrogen fluoride was tested under laboratory conditions over the concentration range of 0.56 – 4.47 mg HF/m. The lowest concentration was chosen to be close to the Polish TLV (0.5 mg HF/m). The dosimeter consists of a Whatman No.1 filter paper impregnated with K2HPO4 placed in a polyethylene holder protected by a polyethylene screen. After ex...

A systematic investigation was conducted to study the effect of paper type on the analytical performance of a series of microfluidic paper-based analytical devices (μPADs) fabricated using a CO2 laser engraver. Samples included three different grades of Whatman chromatography paper, and three grades of Whatman filter paper. According to the data collected and the characterization performed, dif...

Pupply. With a reverse transcription-PCR (RT-PCR) amplifylng heat shock protein 70 (hsp70) gene, we were able to detect two differenttypes of viable Cyyptosporidium oocysts from the environment. Water specimens were filtered under positive pressure through a 3-Elm polycarbonate filter (Nuclepore filter, Whatman, Maidstone, England). The trapped materials were detached from the filter by sonicat...

Circulating anodic antigen (CAA) testing is a powerful, increasingly-used tool for diagnosis of active schistosome infection. We sought to determine the feasibility and reliability of measuring CAA in blood spots collected on Whatman 903 protein saver cards, which are the predominant filter papers used worldwide for dried blood spot (DBS) research and clinical care. CAA was eluted from blood sp...

This paper discusses the potential of paper-based microfluidic devices for affordable diagnostics, and importance flow control. We have explored variation width paper, effect 2D flow, altering surface wettability to increase resistance fluid by physicochemical barriers. used Whatman filter No. 40 1, mustard oil, oleic acid, examine due capillary forces. Moreover, in thickness storage reservoir ...

This protocol describes mosquito rearing in the insectary. The insectary rooms are maintained at 28 degrees C and approximately 80% humidity, with a 12 hr. day/night cycle. For this procedure, you'll need mosquito cages, 10% sterile sucrose solution, paper towels, beaker, whatman filter paper, glass feeders, human blood and serum, water bath, parafilm, distilled water, clean plastic trays, mosq...

The procedure previously described (Zwarenstein, 1949) has been modified as follows. A small knife-point (about 5 mg.) of solid o-tolidine hydrochloride is added to 5 drops of urine in a test-tube and thoroughly mixed. A drop of the mixture is placed on a small piece of Whatman No. 1 filter paper (about 17 mm. square) on a white porcelain tile. The drop is allowed to soak into the paper and one...