Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

The request to see God is one of the theological paradoxes mentioned in the life story of Prophet Moses (a) that apparently opposes his infallibility. The verse 143 of the chapter A’rāf is one of the places this story is narrated. The opponents of infallibility suggest that Moses knew God is not physical or visible, nor occupies a place, so why did he have such a request? The answer is that he ...

We introduce a model of strategic thinking in games of initial response. Unlike standard models of strategic thinking, in this framework the player’s ‘depth of reasoning’ is endogenously determined, and it can be disentangled from his beliefs over his opponent’s cognitive bound. In our approach, individuals act as if they follow a cost-benefit analysis. The depth of reasoning is a function of t...

A sophisticated player is an individual who takes the action of the opponents, in a strategic situation, as determined by decision of rational opponents, and acts accordingly. A sophisticated agent is rational in the choice of his action, but ignores the fact that he is part of a strategic situation. We discuss a notion of equilibrium with sophisticated agents, we provide conditions for its exi...

We propose that individuals use anger strategically in interactions. We first show that in some environments angering people makes them more effective in competitions, whereas in others, anger makes them less effective. We then show that individuals anticipate these effects and strategically use the option to anger their opponents. In particular, they are more likely to anger their opponents wh...

Label-free detection of the interaction between hexahistidine-tagged human κ-opioid receptor membrane protein and anti-His antibody is demonstrated in liquid by an optical microelectromechanical system utilizing electromagnetically actuated microresonators. Shift in resonance frequency due to accretion of mass on the sensitive surface of microresonators is monitored via an integrated optical re...

We evaluate the ability of hexahistidine (His6) tags on peptide and protein substrates to recruit deoxyribozymes for modifying those substrates. For two different deoxyribozymes, one that creates tyrosine-RNA nucleopeptides and another that phosphorylates tyrosine side chains, we find substantial improvements in yield, kobs, and Km for peptide substrates due to recruiting by His6/Cu(2+). Howeve...

Making a decision, an agent must consider how his outcome can be influenced by possible actions of other agents. A ’best defense model’ for games involving uncertainty assumes usually that the opponents know everything about the actual situation and the player’s plans for certain. In this paper it’s argued that the assumption results in algorithms that are too cautious to be good in many game s...