Optically pure â,â-dimethylated amino acid building blocks with functionalized side chains have been prepared from D-aspartic acid. The dimethylation was accomplished by regioselective dialkylation of 9-phenylfluorenyl (PhFl)-protected aspartate diesters. The bulk of the PhFl protecting group also allowed for a variety of functional group manipulations to be carried out on the side chain withou...

The antitumor effect of 22-oxa-calcitriol (OCT), a newly developed noncalcemic analogue of calcitriol, was examined /'/; vivo in athymic mice implanted with human breast carcinoma with or without estrogen recep tor (ER). In ER-positive MCF-7 tumor, the growth of which was depen dent on exogenous estrogen, administration p.o. of OCT as well as the antiestrogen tamoxifen five times a week for 4 w...

We have examined the responses of energy and protein metabolism to nutrient intake in nine patients with lung carcinoma, of whom none »ere cachexie and only one had distant métastases,compared with nine control patients for elective aneurism surgery, who were comparable in terms of age, body mass index, and smoking habits. Whole-body protein turnover and leucine oxidation were assessed by pri...

We have examined the responses of energy and protein metabolism to nutrient intake in nine patients with lung carcinoma, of whom none »ere cachexie and only one had distant métastases,compared with nine control patients for elective aneurism surgery, who were comparable in terms of age, body mass index, and smoking habits. Whole-body protein turnover and leucine oxidation were assessed by pri...

Selective placement of an aliphatic â-alanine (â) residue paired side-by-side with either a pyrrole (Py) or imidazole (Im) aromatic amino acid is found to compensate for sequence composition effects for recognition of the minor groove of DNA by hairpin pyrrole-imidazole polyamides. A series of polyamides were prepared which contain pyrrole and imidazole aromatic amino acids, as well as γ-aminob...

A site-specific labeling method was developed in which sulfhydryl groups of a murine IgG2a anti-ovarian monoclonal antibody, 5G6.4, were biotinylated with /V-iodoacetyl-yV'-biotinylhexylenediamine (Compound /) following partial reduction of disulfide bonds with dithiothreitol. Reaction of 7-alkylated 5G6.4 with 125I-streptavidingave immunoreactive streptavidin-/-biotinylated complexes. Radio-fa...

Objective—To evaluate the role of vitamin A on renal scarring in recurrent urinary tract infections (UTIs). Design—Twenty three children with UTIs and renal scarring (mean (SD) age 7.3 (3.9) years) and 91 children without renal scarring (6.4 (3.4) years) were studied. All the children had serum vitamin A and â-carotene measurements and nutritional evaluation. Renal scarring was assessed by tech...

In protection experiments (5-formyltetrahydrofolate or 5-methyltetrahydrofolate added to cultures in vitro at the same time as methotrexate), 5-methyltetrahydrofolate af forded the same degree of protection as did formyltetrahydrofolate for phytohemagglutinin-stimulated peripheral blood lymphocytes and for methotrexate-resistant (R) and methotrexate-sensitive (S) sublines of CCRF-CEM. Either re...

Hepatic and splenic microsomal electron transport system function, microsomal heme oxygenase activity, and microsomal drug metabolism have been examined in male Wistar rats bearing Murphy-Sturm lymphosarcoma 2 to 15 days following i.m. tumor transplantation. Hepatic microsomal cytochrome P450 and NADPH-cytochrome c reducÃ-ase activity decreased significantly [cytochrome P-450 nadir was 0.49 ± ...

A human immunoglobulin that binds prostatic acid phosphatases (PAP) was isolated from the serum of normal individuals by affinity chromatography using a PAP-Sepharose solid ad sorbent. The yield of isolated protein, termed PAP-binding globulin (PAPBG), ranged from 4.7 to 16.3 /¿g/mlserum. As shown by immunoelectrophoresis, PAPBG is a y-globulin of restricted electrophoretic heterogeneity. PAPB...