Impact of Wheat streak mosaic virus and Triticum mosaic virus on transmission by Aceria tosichella Keifer (Eriophyidae) and virus epidemiology in wheatImpact of Wheat streak mosaic virus and Triticum mosaic virus on transmission by Aceria tosichella Keifer (Eriophyidae) and virus epidemiology in wheat" (2013). The wheat curl mite (WCM), Aceria tosichella Keifer, transmits a complex of viruses, ...

A Wheat streak mosaic virus (WSMV) genome lacking HC-Pro was constructed and confirmed by reverse transcription-PCR to systemically infect wheat, oat, and corn. Coupled in vitro transcription/translation reactions indicated that WSMV P1 proteinase cleaved the polyprotein at the P1/P3 junction of the HC-Pro null mutant. The WSMV HC-Pro null mutant was competent for virion formation, but the viru...

ABSTRACT The complete nucleotide sequence of wheat streak mosaic virus (WSMV) has been determined based on complementary DNA clones derived from the 9,384-nucleotide (nt) RNA of the virus. The genome of WSMV has a 130-nt 5' leader and 149-nt 3'-untranslated region and is polyadenylated at the 3' end. WSMV RNA encodes a single polyprotein of 3,035 amino acid residues and has a deduced genome org...

Wheat streak mosaic virus (WSMV) is an eriophyid mite-transmitted virus of the genus Tritimovirus, family Potyviridae. Complete deletion of helper component-proteinase (HC-Pro) has no effect on WSMV virulence or disease synergism, suggesting that a different viral protein suppresses RNA silencing. RNA silencing suppression assays using Nicotiana benthamiana 16C plants expressing GFP were conduc...

Byamukama, E., Tatineni, S., Hein, G. L., Graybosch, R. A., Baenziger, P. S., French, R., and Wegulo, S. N. 2012. Effects of single and double infections of winter wheat by Triticum mosaic virus and Wheat streak mosaic virus on yield determinants. Plant Dis. 96:859-864. Triticum mosaic virus (TriMV) is a recently discovered virus infecting wheat (Triticum aestivum) in the Great Plains region of...

The development of wheat (Triticum aestivum L.) cultivars that are resistant to Wheat streak mosaic virus (WSMV), yet competitive in yield under nondiseased conditions, is an objective for breeding programs in the Great Plains. This field study was conducted to compare classical and transgenic sources of resistance to WSMV. Three sets of germplasm were evaluated. These included adapted cultivar...

Wheat curl mite (WCM) is the only known arthropod vector of four wheat viruses, most important which streak mosaic virus (WSMV). Host resistance to WCM and WSMV limited a small number loci, are introgressed from wild relatives often associated with linkage drag temperature sensitivity. Reports virulent populations potential resistance-breaking isolates highlight need for more diverse sources re...

Inserts bearing the coding sequences of NPT II and beta-glucuronidase (GUS) were placed between the nuclear inclusion b (NIb) and coat protein (CP) domains of the wheat streak mosaic virus (WSMV) polyprotein ORF. The WSMV NIb-CP junction containing the nuclear inclusion a (NIa) protease cleavage site was duplicated, permitting excision of foreign protein domains from the viral polyprotein. Whea...

Monopartite members of the family Potyviridae utilize three virus-encoded proteinases to cleave the viral polyprotein into mature proteins. The amino-terminal region of the viral polyprotein is autolytically cleaved by the P1 proteinase. A domain required for P1 proteinase activity of Wheat streak mosaic virus (WSMV) was mapped using a series of templates with nested 3'-truncations or 5'-deleti...

Journal of Plant Registrations, Vol. 3, No. 1, January 2009 51 W heat streak mosaic virus (WSMV) is one of the most common, and devastating, diseases impacting wheat (Triticum aestivum L.) production in the western Great Plains of North America. Annual production losses of 5% have been estimated, although severe local WSMV infection can result in complete crop failure (French and Stenger, Regis...