نتایج جستجو برای: xrcc4

تعداد نتایج: 389  

Journal: :Biochemical Society transactions 2011
Qian Wu Takashi Ochi Dijana Matak-Vinkovic Carol V Robinson Dimitri Y Chirgadze Tom L Blundell

XRCC4 (X-ray cross-complementation group 4) and XLF (XRCC4-like factor) are two essential interacting proteins in the human NHEJ (non-homologous end-joining) pathway that repairs DNA DSBs (double-strand breaks). The individual crystal structures show that the dimeric proteins are homologues with protomers containing head domains and helical coiled-coil tails related by approximate two-fold symm...

2008
Leonie Schulte-Uentrop Raafat A. El-Awady Lena Schliecker Henning Willers Jochen Dahm-Daphi

Non-homologous end-joining (NHEJ) of DNA double-strand breaks (DSBs) is mediated by two protein complexes comprising Ku80/Ku70/DNA-PKcs/Artemis and XRCC4/LigaseIV/XLF. Loss of Ku or XRCC4/LigaseIV function compromises the rejoining of radiation-induced DSBs and leads to defective V(D)J recombination. In this study, we sought to define how XRCC4 and Ku80 affect NHEJ of site-directed chromosomal ...

2016
Manabu KOIKE Yasutomo YUTOKU Aki KOIKE

Various chemotherapies and radiation therapies are useful for killing cancer cells mainly by inducing DNA double-strand breaks (DSBs). Uncovering the molecular mechanisms of DSB repair processes is crucial for developing next-generation radiotherapies and chemotherapeutics for human and animal cancers. XRCC4 plays a critical role in Ku-dependent nonhomologous DNA-end joining (NHEJ) in human cel...

2014
Shoji IMAMICHI Mukesh Kumar SHARMA Radhika Pankaj KAMDAR Mikoto FUKUCHI Yoshihisa MATSUMOTO

XRCC4 (X-ray cross-complementation group 4) is a protein associated with DNA ligase IV, which is thought to join two DNA ends at the final step of DNA double-strand break repair through non-homologous end-joining. It has been shown that, in response to irradiation or treatment with DNA damaging agents, XRCC4 undergoes phosphorylation, requiring DNA-PK. Here we explored possible role of ATM, whi...

2016
Mukesh Kumar Sharma Shoji Imamichi Mikoto Fukuchi Ravindra Mahadeo Samarth Masanori Tomita Yoshihisa Matsumoto

XRCC4 is a protein associated with DNA Ligase IV, which is thought to join two DNA ends at the final step of DNA double-strand break repair through non-homologous end joining. In response to treatment with ionizing radiation or DNA damaging agents, XRCC4 undergoes DNA-PK-dependent phosphorylation. Furthermore, Ser260 and Ser320 (or Ser318 in alternatively spliced form) of XRCC4 were identified ...

Journal: :The EMBO journal 2000
M S Junop M Modesti A Guarné R Ghirlando M Gellert W Yang

XRCC4 is essential for carrying out non-homologous DNA end joining (NHEJ) in all eukaryotes and, in particular, V(D)J recombination in vertebrates. Xrcc4 protein forms a complex with DNA ligase IV that rejoins two DNA ends in the last step of V(D)J recombination and NHEJ to repair double strand breaks. XRCC4-defective cells are extremely sensitive to ionizing radiation, and disruption of the XR...

Journal: :The EMBO journal 2004
Christine Anne Koch Roger Agyei Sarah Galicia Pavel Metalnikov Paul O'Donnell Andrei Starostine Michael Weinfeld Daniel Durocher

Nonhomologous end joining (NHEJ) is the major DNA double-strand break (DSB) repair pathway in mammalian cells. A critical step in this process is DNA ligation, involving the Xrcc4-DNA ligase IV complex. DNA end processing is often a prerequisite for ligation, but the coordination of these events is poorly understood. We show that polynucleotide kinase (PNK), with its ability to process ionizing...

Journal: :Nucleic acids research 1998
E B Kabotyanski L Gomelsky J O Han T D Stamato D B Roth

The Ku86 and XRCC4 proteins perform critical but poorly understood functions in the repair of DNA double-strand breaks. Both Ku 86- and XRCC4-deficient cells exhibit profound radiosensitivity and severe defects in V(D)J recombination, including excessive deletions at recombinant junctions. Previous workers have suggested that these phenomena may reflect defects in joining of the broken DNA ends...

Journal: :Human molecular genetics 2015
Nadine Rosin Nursel H Elcioglu Filippo Beleggia Pinar Isgüven Janine Altmüller Holger Thiele Katharina Steindl Pascal Joset Anita Rauch Peter Nürnberg Bernd Wollnik Gökhan Yigit

DNA double-strand breaks (DSBs) are highly toxic lesions, which, if not properly repaired, can give rise to genomic instability. Non-homologous end-joining (NHEJ), a well-orchestrated, multistep process involving numerous proteins essential for cell viability, represents one major pathway to repair DSBs in mammalian cells, and mutations in different NHEJ components have been described in microc...

Journal: :Cancer research 2003
Jae Wan Lee Steven M Yannone David J Chen Lawrence F Povirk

In the nonhomologous end joining pathway of DNA double-strand break repair, the ligation step is catalyzed by a complex of XRCC4 and DNA ligase IV. Extracts of CHO-K1 cells are able to accurately rejoin a site-specific free radical-mediated double-strand break with partially complementary overhangs, by a mechanism involving alignment-based gap filling followed by ligation. Extracts of XR-1 cell...

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