نتایج جستجو برای: yeast expression vector

تعداد نتایج: 1122471  

Journal: :journal of sciences, islamic republic of iran 2003
p. mousavi shafaee

interferon is a protein secreted by eucaryotic cells following stimulation by viruses, bacteria, and many other immunogenes. recent medical studies indicate that interferons have effective role in the treatment of virus infections, immunodeficiency and certain types of cancer such as hairy cell leukaemia (hcl). the aim of the present study is to apply yeast strain for secreting human ifnα2b fol...

Journal: :medical journal of islamic republic of iran 0
ali a farhoudi-moghaddam from the molecular biology unit . pasteur institute. tehran. i.r. iran nickolay n domansky a.a ziaee

the genome of hb v virus of serotype ayw cloned in pbr322 and expression shuttle vector p yes2 were used for construction of the hbsag chimeric genes and their expression in saccharomyces cerevisiae. two recombinant plasmids were constructed. one of them contained the coding sequences for the major polypeptide of surface antigen. another construct carried the major polypeptide with the pre-s2 a...

Journal: :کومش 0
محمدرضا اکبری عیدگاهی m.r. akbari eidgahi biotechnology researches center, semnan university of medical sciences, semnan, iranدانشگاه علوم پزشکی سمنان، مرکز تحقیقات بیوتکنولوژی رضا نصر r. nasr dept. of microbiology, azad university of jahrom, jahrom, iranدانشگاه آزاد اسلامی جهرم، گروه میکروبیولوژی علی اکبر شعبانی a.a. shaebani biotechnology researches center, semnan university of medical sciences, semnan, iranدانشگاه علوم پزشکی سمنان، مرکز تحقیقات بیوتکنولوژی مجید مقبلی m. moghbeli dept. of microbiology, azad university of damqan, damgan, iranدانشگاه آزاد اسلامی دامغان، گروه میکروبیولوژی

introduction: follicle stimulating hormone (fsh) is one of the pituitary glycoproteines that it consists of two subunits alpha and beta. the beta subunit is responsible for the biological activity of fsh. the aim of present study was isolation of the β subunit coding sequence containing its signal sequence from human genome and then cloning of the isolated sequence in ppic9 shuttle vector under...

Journal: :iranian journal of biotechnology 2007
ali bahrami seyed abbas shojaosadati rasoul khalilzadeh ali reza saeedinia ebrahim vasheghani farahani

human granulocyte-colony stimulating factor (hg-csf) cdna was expressed in the methylotrophic yeast pichia pastoris under the control of the alcohol oxidase (aox1) promoter. an expression vector for hg-csf secretion was constructed using vector ppic9. higher levels of hg-csf was obtained using a p. pastoris mut+ (methanol utilization fast) phenotype. the effects of environmental factors such as...

Ali Bahrami Ali Reza Saeedinia Ebrahim Vasheghani Farahani, Jafar Mohammadian-Mosaabadi Rasoul Khalilzadeh Seyed Abbas Shojaosadati,

Human granulocyte-colony stimulating factor (hG-CSF) cDNA was expressed in the methylotrophic yeast Pichia pastoris under the control of the alcohol oxidase (AOX1) promoter. An expression vector for hG-CSF secretion was constructed using vector pPIC9. Higher levels of hG-CSF was obtained using a P. pastoris Mut+ (methanol utilization fast) phenotype. The effects of environmental factors such as...

Journal: :avicenna journal of medical biotechnology 0

to accomplish the worldwide demand for recombinant human erythropoietin (rhuepo) as a therapeutic, application of cost-efficient expression system of methylotrophic yeast pichia pastoris (p. pastoris) rather than mammalian cells is indispensable. herein, a report on high levels secreted-expression of pichia-derived rhuepo by batch fermentation in a ph stabilized format is presented. the full le...

Journal: :avicenna journal of medical biotechnology 0

the green fluorescent protein (gfp) was originally isolated from the jellyfish aequorea victoria that fluoresces green when exposed to blue light. gfp protein is composed of 238 amino acids with the molecular mass of 26.9 kd. the gfp gene is frequently used in cellular and molecular biology as a reporter gene. to date, many bacterial, yeast , fungal, plants, fly and mammalian cells, including h...

Journal: :jundishapur journal of microbiology 0
fahimeh afzal-javan department of genetics, faculty of science, university of shahrekord, shahrekord, ir iran; research institute of biotechnology, university of shahrekord, shahrekord, ir iran mohsen mobini-dehkordi department of genetics, faculty of science, university of shahrekord, shahrekord, ir iran; research institute of biotechnology, university of shahrekord, shahrekord, ir iran; department of genetics, faculty of science , university of shahrekord, shahrekord, ir iran. tel/fax. +98-3814424419

conclusions based on this similarity and our bioinformatics evaluations, this mentioned alpha-amylase gene can be expressed in s. cerevisiae as extracellular enzyme. results the presence of alpha-amylase gene in recombinant bacteria was certificated by colony-pcr method. after extraction of recombinant vector from e. coli, the competent s. cerevisiae cells were transformed using polyethylene gl...

Journal: :avicenna journal of medical biotechnology 0

human alpha 1-antitrypsin (aat) cdna was obtained from hepg2 cell lines. after pcr and construction of expression vector ppiczα-aat, human aat was expressed in the yeast pichia pastoris (p.pastoris) in a secretary manner and under the control of inducible alcohol oxidase 1 (aox1) promoter. the amount of aat protein in medium was measured as 60 mg/l 72 hr after induction with methanol. results i...

Journal: :journal of biotechnology and health sciences 0
afshin rostami department of plant protection, university of zanjan, zanjan, ir iran; department of plant protection, university of zanjan, zanjan, ir iran. tel/fax: +98-2632238529 nemat sokhandan bashir department of plant protection, university of tabriz, tabriz, ir iran parviz pirniakan department of agriculture, payame noor university, ir iran nahid masoudi department of plant protection, university of guilan, rasht, ir iran

background cucumber mosaic virus (cmv) has isometric particles with a diameter of about 28–29 nm. a useful method to recognize regions from mutagenesis in the coat protein (cp) gene are important for particle assembly and the expression of viral in cp expression systems like bacteria such as e.coli or yeast as well as their assembly into virus-like particles (vlp). objectives in this paper, we ...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید