The wine yeast, Saccharomyces cerevisiae, is the best understood microbial eukaryote at the molecular and cellular level, yet its natural geographic distribution is unknown. Here we report the results of a field survey for S. cerevisiae,S. paradoxus and other budding yeast on oak trees in Europe. We show that yeast species differ in their geographic distributions, and investigated which ecologi...

 Abstract Background: The disk diffusion method for identification of yeasts species was performed based on different but distinct susceptibilities of yeasts spp. to chemicals: janus green, ethidium bromide, 2,3,5-triphenyltetrazolium chloride, brilliant green, cycloheximide and rhodamine 6G. Methods: Atotal of 568 Iranian soldiers went under study for isolation and identification of Yeast spec...

A method for isolation of nuclei from Saccharomyces cervisiae in high yield is described. The DNA/protein ratio of the isolated nuclei is 10 times higher than that of whole cells. Examination of these nuclei in phase and electron microscopes has shown them to be round bodies having a double membrane, microtubules, and a dark crescent at one end. The optimum conditions for extraction and resolut...

The yeast two-hybrid system is frequently used to identify protein-protein interactions. Confirming the specificity of candidate clones requires separation and isolation of yeast plasmids, propagation in bacteria and testing combinations of DNA-binding and activation domain hybrids in yeast. In order to simplify this procedure, we developed a rapid method based on PCR amplification of library i...

Tetraploid cultures of Saccharomyces cerevisiae triplex (A/A/A/a) at several loci were sporulated. All the diploid spores are expected to be homozygous (A/A) or heterozygous (A/a) and, hence, to have the dominant phenotype. Cells lacking one of the chromosomes (monosomics) may show the recessive phenotype (a/-). Therefore, spores that grew on complete medium but failed to grow on the relevant s...

Any proteomics analysis starts with sample preparation. For organelle proteomics, where the aim is the identification of organelle-specific proteins, the sample preparation involves the isolation of an organelle of interest to a high degree of purity devoid of cross-contaminations from other organelles. Different organelles of the yeast Saccharomyces cerevisiae possess unique physical/chemical ...

Because of the high energy consumption for fermentor cooling, the isolation of thermo-tolerant Acetobacter strains for vinegar production has a high priority. The aims of this study were the isolation and identification of a high ethanol-thermo-tolerant Acetobacter spp. from grapes as well as the optimization of conditions for increasing the acetic acid production. The grape e...

Because of the high energy consumption for fermentor cooling, the isolation of thermo-tolerant Acetobacter strains for vinegar production has a high priority. The aims of this study were the isolation and identification of a high ethanol-thermo-tolerant Acetobacter spp. from grapes as well as the optimization of conditions for increasing the acetic acid production. The grape e...