microscopy as “field based test” was compared with is900 blood pcr for the diagnosis and estimationof prevalence of mycobacterium avium subspecies paratuberculosis (map) in domestic livestock withclinical johne’s disease (jd). of 252 animals screened, 39.3 and 13.1% were positive by faecal microscopyand blood pcr, respectively. proportional agreement between “microscopy” and blood pcr was subst...

Objective. To study the correlation of cytomorphological features in fine needle aspiration smears from patients suspected of having tuberculous lymphadenitis with Ziehl-Neelsen staining (ZN), auramine-rhodamine staining (ARS), and autofluorescence (AF). Methods. A total of 145 lymph nodes were aspirated, 3 air-dried smears were stained with Giemsa, Ziehl-Neelsen, and auramine-rhodamine stains,...

Thirty six tissues from sheep, previously diagnosed with paratuberculosis, were tested by PCR in positive Ziehl-Neelsen staining smears of tissues, and PCR in tissues targeting IS900 specific for Mycobacterium avium subsp. paratuberculosis. DNA amplification was achieved in 33.3% Ziehl-Neelsen smears, and in 61.1% tissue samples. Combination of both techniques found 66.7% samples as positive. C...

INTRODUCTION Patients with human immunodeficiency virus carry a significant risk of contracting opportunistic infections. The worldwide increased incidence of tuberculosis has instituted pulmonary tuberculosis as an important diagnostic consideration in patients with human immunodeficiency virus presenting with lower respiratory tract infection. A positive result on the readily-available Ziehl-...

BACKGROUND We evaluated the Ziehl-Neelsen staining (ZNS) technique for the diagnosis of paragonimiasis in Laos and compared different modifications of the ZNS techniques. METHODOLOGY WE APPLIED THE FOLLOWING APPROACH: We (1) examined a paragonimiasis index case's sputum with wet film direct examination (WF) and ZNS; (2) re-examined stored ZNS slides from two provinces; (3) compared prospectiv...

Thirty faecal specimens from 22 patients with the acquired immune deficiency syndrome were examined by microscopy after Ziehl-Neelsen staining and by culture after decontamination with sodium hydroxide. Thirteen specimens (from 11 patients) were positive for Mycobacterium avium-intracellulare-scrofulaceum on culture, and only five of these on Ziehl-Neelsen staining. Five of the 11 patients had ...

The purpose of our survey is to estimate the frequency Cryptosporidium spp in dairy calves under 60 days age, pathogenic role development diarrhea, intervention and association other enteropathogens often involved neonatal diarrhea possible relationship between infection certain farming practices order share risk factors, on hand, compare modified Ziehl-Neelsen staining method copro-ELISA test....

Although inexpensive and easy to perform, the negative staining technique of Heine for the detection of Cryptosporidium spp. has been largely neglected. The lack of familiarity with the negative staining technique, the misconception that a phase-contrast microscope is indispensible and inferior results due to Köhler-illumination, are possible explanations for its low popularity. While the modif...