Rudgers and Sastry-Dent Approaches to improving agricultural crops have relied on either mutational or traditional breeding and selection methods or through the random introduction of novel genes into crops by transformation (e.g. using Agrobacterium). These approaches are inherently time-consuming, inefficient and expensive, especially for developing complex, multi-trait products that are now ...

Abbreviations: rAAV, recombinant adeno-associated virus; DNA, deoxyribonucleic acid; DSBs, doublestrand breaks; SDSA, synthesis dependent strand annealing; ES, embryonic stem; ESC, embryonic stem cell; HPRT, hypoxanthine phosphoribosyltransferase; HAT, hypoxanthine-aminopterin-thymidine; ITR, inverted terminal repeat; bp, basepair; ZFN, zinc finger nuclease; SCID, severe combined immunodeficien...

Background In vivo multi-modality cellular and molecular imaging of the engrafted iCMs is necessary to characterize the engraftment and the regional effects on the viability of the injured myocardium. Zinc finger nuclease (ZFN)mediated integration of the reporter gene into the AAVS1 locus in the iCMs and manganese enhanced MRI (MEMRI) should allow precise in vivo detection of myocardial regener...

Engineered nucleases have been used to generate knockout or reporter cell lines and a range of animal models for human disease. These new technologies also hold great promise for therapeutic genome editing. Current methods to evaluate the activity of these nucleases are time consuming, require extensive optimization and are hampered by readouts with low signals and high background. We have deve...

Zinc finger nuclease (ZFN) is a useful tool for endogenous site-directed genome modification. The development of an easier, less expensive and repeatedly usable construction method for various sequences of ZFNs should contribute to the further widespread use of this technology. Here, we establish a novel construction method for ZFNs. Zinc finger (ZF) fragments were synthesized by PCR using shor...

Engineered nucleases have been used to generate knockout or reporter cell lines and a range of animal models for human disease. These new technologies also hold great promise for therapeutic genome editing. Current methods to evaluate the activity of these nucleases are time consuming, require extensive optimization and are hampered by readouts with low signals and high background. We have deve...

The polyadenylation of messenger RNAs is mediated by a multi-subunit complex that is conserved in eukaryotes. Among the most interesting of these proteins is the 30-kDa-subunit of the Cleavage and Polyadenylation Specificity Factor, or CPSF30. In this study, the Arabidopsis CPSF30 ortholog, AtCPSF30, is characterized. This protein possesses an unexpected endonucleolytic activity that is apparen...