Glu(282) located in the NH(2)-terminal part of transmembrane helix M3 of the Na(+),K(+)-ATPase was replaced by alanine, glycine, leucine, lysine, aspartate, or glutamine, and the effects of the mutations on the overall and partial reactions of the enzyme were analyzed. The mutations affected at least 3 important functions of the Na(+),K(+)-ATPase: (i) the conformational transitions between E(1)...

Orthodontics has been developing for 282 years. We can distinguish an initial period when practitioners limited themselves to ‘‘straightening’’ teeth that were out of position. Then, under the influence of Edward Hartley Angle, they devoted their efforts to correcting ‘‘malocclusions.’’ Now, during its third period, orthodontics has divided itself into two branches. The American school believes...

Abstract Background COVID-19 vasculopathy is a critical condition that impacts the disease prognosis including vasculitis and thromboembolic complications. This study aimed to provide Egyptian experience about during past two years of pandemic collectively include different modalities imaging techniques for diagnosis cerebrovascular, pulmonary, gastrointestinal, peripheral arterial vascular Res...

The first microRNAs were discovered some 20 years ago, but only a small fraction of the microRNA-encoding genes have been described in detail yet. Here we report the molecular analysis of a computationally predicted Drosophila melanogaster microRNA gene, mir-282. We show that the mir-282 gene is the source of a 4.9-kb-long primary transcript with a 5' cap and a 3'-poly(A) sequence and a mature ...

Secchi depth has been measured in Lake Tahoe an average of every 12 d since July 1967. Because of the unusual clarity of the lake, Secchi depth measurement is responsive to small changes in light-attenuating particles, and the record exhibits strong variability at the seasonal, interannual, and decadal scales. Using recently developed methods of applied time-series analysis, the mechanisms of c...

The depressant effect of 5-chloro-4-(2-imidazolin-2-yl-amino)-2,1,3-benzothiodiazole (DS103-282) on the polysynaptic excitation of interneurones in the cat spinal cord appears to be related to a postsynaptic reduction in the effectiveness of excitatory transmitters than to interference with their presynaptic release.

RATIONALE Cardiac myosin-binding protein-C (cMyBP-C) phosphorylation at Ser-273, Ser-282, and Ser-302 regulates myocardial contractility. In vitro and in vivo experiments suggest the nonequivalence of these sites and the potential importance of Ser-282 phosphorylation in modulating the protein's overall phosphorylation and myocardial function. OBJECTIVE To determine whether complete cMyBP-C p...

4. Automated Interpretation of CID Spectra 282 5. Accurate Mass Tags 282 C. Protein Identification in Complex Mixtures 282 D. Analysis of Protein Expression 284 III. Proteomes and Post-Translational Modifications 285 A. Proteomes 285 1. The Analytical Challenge 285 2. Analysis of Protein−Protein Complexes 286 B. Post-Translational Modifications 286 1. Background 286 2. Detection and Purificatio...

Restriction of virus-specific cytotoxic T cell function by products of the major histocompatability complex (MHC), 1 a phenomenon first described in murine systems (1-3), has now been confirmed for the human T cell responses to antigens on cells infected by influenza (4, 5), Epstein-Barr (EB) (6, 7), and more recently, mumps (8), measles (9), and cytomegalo(10) 2 viruses. In each case the patte...

Objective: To examine the relationship between child maltreatment, clinically relevant adjustment problems, and dating violence in a community sample of adolescents. Method: Adolescents from 10 high schools (N = 1,419; response rate = 62%) in southwestern Ontario completed questionnaires that assessed past maltreatment, current adjustment, and dating violence. Logistic regression was used to co...