Chronic infection with Toxoplasma gondii induces a potent resistance against reinfection, and IFN-γ production by CD8(+) T cells is crucial for the protective immunity. However, the molecular mechanisms that regulate the secondary response remain to be elucidated. In the current study, we examined the role of IL-2 in IFN-γ production by CD8(+) immune T cells in their secondary responses using T...

OBJECTIVE To observe the discrepancies of responses induced by Schistosoma japonicum (S. japonicum) normal cercaria antigen (NCA) and ultraviolet (UV) -radiation-attenuated cercaria antigen (UVACA) in an in vitro system. METHODS S. japonicum cercariae were collected and UVACA and NCA were prepared. Mouse macrophage model cells (RAW 264.7) were treated with medium, NCA (40 µg/mL) or UVACA (40 ...

background: dendritic cells (dc) are a key regulator of the immune response, and interferon- beta (ifn-β) is considered an immunomodulatory molecule for dc. objective: the purpose of this study was to evaluate the ability of ifn-β treated dc to induce cytokine secretion by cd4+ t cells. methods: dendritic cells were generated from blood monocytes with granulocyte-monocyte colony-stimulating fac...

Umbelliprenin is a sesquiterpene coumarin with vitro anti-carcinogenic activities. The aim of this study was to investigate the antitumor effects of umbelliprenin in animal models of colorectal cancer. The cytotoxic effects of umbelliprenin were explored on CT26 and L929by MTT assay. In this study, colorectal tumors developed in mice by intradermal injection of CT26 cell line. Tumor size, serum...

Umbelliprenin is a sesquiterpene coumarin with vitro anti-carcinogenic activities. The aim of this study was to investigate the antitumor effects of umbelliprenin in animal models of colorectal cancer. The cytotoxic effects of umbelliprenin were explored on CT26 and L929by MTT assay. In this study, colorectal tumors developed in mice by intradermal injection of CT26 cell line. Tumor size, serum...

Background: Despite demonstration of molecular and biochemical changes induced by heat shock on Leishmania, the immunological importance of such changes has not been elucidated.  Objective: Studying the effect of two excretory factors prepared under heat shock and ambient temperature from Leishmania major on Balb/c splenocytes function.  Methods: The parasites were cultured at 25°C and then sub...

A major limitation of most animal models of autoimmune diseases is that they do not reproduce the chronic or relapsing-remitting pattern characteristic of many human autoimmune diseases. This problem has been overcome in our rat models of experimentally induced monophasic or relapsing-remitting autoimmune uveitis (EAU), which depend on the inducing antigen peptides from retinal S-Antigen (monop...

Sunto. Si prova che il Γ-limite in Lμ di una successione di funzionali supremali della forma Fk(u) = μ-ess supΩ fk(x, u) é un funzionale supremale. In un controesempio si mostra che la funzione che rappresenta il Γ-limite F (·, B) di una successione di funzionali supremali della forma Fk(u,B) = μ-ess supB fk(x, u) puó dipendere dall’insieme B e si stabilisce una condizione necessaria e sufficie...

background: generation of an effective dendritic cell (dc) based cancer vaccine depends on appropriate differentiation of monocytes in vitro. objective: to compare the effects of monocyte separation methods, flask adherence (flask-dc) and magnetic activated cell sorting (macs-dc), on phenotypic and functional characteristics of resultant dcs. methods: dcs from healthy volunteers were generated ...