長年，アレルゲンの解析手法としてSDS-PAGEとWestern blottingが用いられてきた．この場合，タンパク質をできるだけ可溶化するために，SDSなどの変性剤を高濃度で添加することが多い．筆者らはこの手法を用いて，甲殻類アレルゲンの種特異的反応性の違いを検出した．ところが，別の患者血清では，この方法ではバンドが全く検出されなかった．そこでこの患者血清をdot blottingで調べたところ，生では強く反応したが，80°Cで15分加熱すると反応が失われた．そこで，生の試料をdot blotしたPVDF膜を0.1％SDSを含む電気泳動バッファー中で1時間インキュベートしたところ反応性は弱まったが，完全には反応が消失していなかった．そこで，0.1％SDSを含むSDS抽出液を用いてSDS-PAGEを行ったところ，トロポミオシンのバンドが弱く反応した．甲殻類アレルゲンのSDS変性を低...

SDS-PAGE was applied to determine trypsin activity and inhibition. After the hydrolysis by trypsin to substrate bovine serum albulnin (BSA) under different temperatures and pH, the hydrolysis degree of BSA was conducted using SDS-PAGE. From the quantitative analysis to the electrophoresis bands of BSA and its hydrolysis products in SDS-PAGE pattern, the change of trypsin activity was determined...

The present study aimed to investigate the effect of N-Ethylmaleimide (NEM) on evaluation disulfide formation in oxi dized myofibrillar proteins during sample preparation non-reducing SDS-PAGE procedure. For this purpose, extracted were oxidized firstly via a Fenton oxidation reaction, and non-oxidized used as control. Before running SDS-PAGE, preparation, these prepared according three dif fer...

INTRODUCTION MATERIALS AND METHODS RESULTS AND DISCUSSION CONCLUSIONS REFERENCES

Our objectives were to determine if milk casein as a percentage of true protein (CN%TP) estimated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) is equivalent CN%TP Kjeldahl, and the proportion (CN), proteolysis products (CNPP), serum (SP) from (TP) that goes into Kjeldahl noncasein nitrogen (NCN) filtrate stays in NCN precipitate using SDS-PAGE. Raw samples collected 1...

An alternative electrophoretic method that improves the separation, identification and relative quantification of bovine milk casein fractions and casein hydrolysates was developed. The method is based in the Tricine-SDS-PAGE technique while the relative quantification of milk proteins has been achieved by digital densitometry. The advantages of this procedure for milk proteins and milk protein...

Abstract The objective of this work was to evaluate microfluidic chip electrophoresis, known as lab-on-a-chip technique, for the detection milk adulteration using cheese whey in comparison with SDS-PAGE. Raw, pasteurized, processed at an ultra-high temperature (UHT), and powdered samples received increasing concentrations (0, 1, 2.5, 5, 10, 20, 30, 50, 100% v/v), were subjected electrophoresis ...