Retinitis pigmentosa (RP) is a group of genetically heterogeneous, severe retinal diseases commonly leading to legal blindness. Mutations in the CNGB1a subunit of the rod cyclic nucleotide-gated (CNG) channel have been found to cause RP in patients. Here, we demonstrate the efficacy of gene therapy as a potential treatment for RP by means of recombinant adeno-associated viral (AAV) vectors in t...

Activation of cyclic nucleotide-gated (CNG) ion channels involves a conformational change in the channel protein referred to as the allosteric transition. The amino terminal region and the carboxyl terminal cyclic nucleotide-binding domain of CNG channels have been shown to be involved in the allosteric transition, but the sequence of molecular events occurring during the allosteric transition ...

Air pollution kills more than 5.9 million people annually, with more than 90 per cent of these deaths in capital city of India, Delhi. For improving the status of air pollution in Delhi, various policies and laws have been implemented. But even after the implementation of CNG, there was no significant change of pollutants (NOx, O3, SPM, RSPM & CO) except SO2. The objective of our study is wheth...

The kinetics of the photocurrent in both rod and cone retinal photoreceptors are independent of membrane voltage over the physiological range (-30 to -65 mV). This is surprising since the photocurrent time course is regulated by the influx of Ca(2+) through cGMP-gated ion channels (CNG) and the force driving this flux changes with membrane voltage. To understand this paradigm, we measured Pf, t...

Compressed Natural Gas (CNG) is an affordable fuel with a higher octane number. However, older CNG kits without electronic controls have the potential to supply more when driving downhill due vacuum in intake manifold. Therefore, this article presents development of control system that accommodates road inclination angles improve efficiency. Machine learning involved work process engine speed, ...

The one dimension computational model of a sequential injection engine, which runs on compressed natural gas (CNG) with spark ignition, is developed for this study, to simulate the performance of gas flow pressure profile, under various speed conditions. The computational model is used to simulate and study of the steady state and transient processes of the intake manifold. The sequential injec...

The aim of the investigation was to prepare nimesulide emulsion for incorporation in Aloe vera gel base to formulate 'nimesulide - Aloe vera transemulgel' (NAE) and to carryout in-vitro assessment and in-vivo anti-inflammatory studies of the product. Although the use of nimesulide is banned for oral administration, due to its potential for inducing hepatotoxicity and thrombocytopenia, the use o...

We examine the economic and environmental implications of the fuels and propulsion technologies that will be available over the next two decades for powering a large proportion of the light duty fleet (cars and light trucks). Since R&D change is rapid, we treat the uncertainty about future technologies using bounding calculations. A lifecycle perspective is used to analyze fossil fuels [convent...

Insulin-like growth factor receptor 1 (IGF-1R)-targeting therapies are currently at an important crossroad given the low clinical response rates seen in unselected patients. Predictive biomarkers for patient selection are critical for improving clinical benefit. Coupling in vitro sensitivity testing of BMS-754807, a dual IGF-1R/IR inhibitor, with genomic interrogations in 60 human colorectal ca...

Apparent blockage of monovalent cation currents by the permeating blocker Ca(2+) is a physiologically essential phenomenon relevant to cyclic nucleotide-gated (CNG) channels. The recently determined crystal structure of a bacterial homolog of CNG channel pores, the NaK channel, revealed a Ca(2+) binding site at the extracellular entrance to the selectivity filter. This site is not formed by the...