Elesgaray R, Caniffi C, Savignano L, Romero M, Mac Laughlin M, Arranz C, Costa MA. Renal actions of atrial natriuretic peptide in spontaneously hypertensive rats: the role of nitric oxide as a key mediator. Am J Physiol Renal Physiol 302: F1385–F1394, 2012. First published February 29, 2012; doi:10.1152/ajprenal.00624.2011.—Atrial natriuretic peptide (ANP) is an important regulator of blood pre...

Chronic exposure to lead (Pb) affects neural functions in central nervous system (CNS) particularly the learning and memory. On the other hand, alteration of calcium level in the CNS results in activation of NOS. It has been shown that lead enters the neurons through calcium channels and displaces Ca2+ from calcium binding proteins such as calmodulin and troponin C thereby affecting calcium-med...

The effects of hypoxia on the regulation of inducible nitric oxide synthase (NOS) 2 expression were examined in cultured rat pulmonary microvascular endothelial cells (EC). EC did not express NOS 2 mRNA or protein when exposed to normoxia or hypoxia unless they were pretreated with interleukin (IL)-1beta and/or tumor necrosis factor (TNF)-alpha for 24 h. Induction of NOS 2 by IL-1beta+TNF-alpha...

Epidermal growth factor receptor (EGFR) is activated by ionizing radiation (IR), but the molecular mechanism for this effect is unknown. We have found that intracellular generation of nitric oxide (NO) by NO synthase (NOS) is required for the rapid activation of EGFR phosphorylation by IR. Treatment of A549 lung cancer cells with IR increased NOS activity within minutes, accompanied by an incre...

Agmatine, decarboxylated arginine, is a metabolic product of mammalian cells. Considering the close structural similarity between L-arginine and agmatine, we investigated the interaction of agmatine and nitric oxide synthases (NOSs), which use L-arginine to generate nitric oxide (NO) and citrulline. Brain, macrophages and endothelial cells were respectively used as sources for NOS isoforms I, I...

AIM To study correlation between inhibitions of naloxone-precipitated withdrawal jumps and nitric-oxide synthase (NOS) activity by agmatine. METHODS NOS activities in mouse brain were measured by determination of concentration of [3H]citrulline, the product of [3H]arginine. RESULTS Agmatine inhibited NOS activity in naive and morphine-dependent mouse cerebellum, forebrain, and thalamus in s...

Mechanisms that regulate the nitric oxide synthase enzymes (NOS) are of interest in biology and medicine. Although NOS catalysis relies on domain motions, and is activated by calmodulin binding, the relationships are unclear. We used single-molecule fluorescence resonance energy transfer (FRET) spectroscopy to elucidate the conformational states distribution and associated conformational fluctu...

Nitric oxide synthase like enzyme (NOS-like enzyme), which produces nitric oxide, participates in many biological processes. However it remains unidentified and highly controversial that plants do possess a NOS-like enzyme. In this paper, a novel arginine analogue NP1 was designed and developed for the direct identification and real time tracking of NOS-like enzymes in plant by fluorescence sen...

pmmSe. The conversion of L-arginine into L-citrulline by nitric oxide synthase (NOS) leads to nitric oxide formation. Two major NOS isoforms exist, a &*-dependent NOS (cNOS) and a &“-independent NOS (iNOS). The cNOS is constitutive of many cells while the iNOS is not expressed in normal conditions but only induced after the stimulation by different factors (endotoxins, cytokines, etc...). This ...

pmmSe. The conversion of L-arginine into L-citrulline by nitric oxide synthase (NOS) leads to nitric oxide formation. Two major NOS isoforms exist, a &*-dependent NOS (cNOS) and a &“-independent NOS (iNOS). The cNOS is constitutive of many cells while the iNOS is not expressed in normal conditions but only induced after the stimulation by different factors (endotoxins, cytokines, etc...). This ...