OBJECTIVE To validate a multiplex polymerase chain reaction (PCR) assay capable of simultaneously screening vitreous biopsy specimens for a panel of common pathogens in posterior uveitis. METHODS A multiplex PCR assay using novel primer sets for cytomegalovirus (CMV), herpes simplex virus (HSV), varicella zoster virus (VZV), and Toxoplasma gondii was developed. The sensitivity of the assay wa...

This paper focuses on the problem of growing multiplex networks. Currently, the results on the joint degree distribution of growing multiplex networks present in the literature pertain to the case of two layers and are confined to the special case of homogeneous growth and are limited to the state state (that is, the limit of infinite size). In the present paper, we first obtain closed-form sol...

Probe-based PCR is widely used for SNP (single nucleotide polymorphism) genotyping and pathogen nucleic acid detection due to its simplicity, sensitivity and cost-effectiveness. However, the multiplex capability of hydrolysis probe-based PCR is normally limited to one target (pathogen or allele) per fluorescence channel. Current fluorescence PCR machines typically have 4-6 channels. We present ...

This research examines how multiplex organizational networks coevolve. Evolutionary and network theories were used to develop hypotheses about tie formation in both uniplex and multiple networks in organizational communities. Based on data between 1997 and 2005 collected from the records of development projects in the field of Information and Communication Technology for Development, the study ...

Wuchereria bancrofti, Dirofilaria immitis, and Dirofilaria repens are filarial nematodes transmitted by mosquitoes belonging to Culex, Aedes, and Anopheles genera. Screening by vector dissection is a tiresome technique. We aimed to screen filarial parasites in their vectors by single and multiplex PCR and evaluate the usefulness of multiplex PCR as a rapid xenomonitoring and simultaneous differ...

This study firstly developed a multiplex real-time PCR (RT-PCR) technique combined with a pre-enrichment step to simultaneously detect Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes) and Salmonella spp. in raw milk and the dairy farm environment (feces, soil, feed, water) in one reaction. Brain heart infusion (BHI) broth was selected for the enrichment step to incre...

هدف: با توجه به رشد روزافزون آموزش شیوه مجازی و ویژه در شرایط گسترش پاندمی کرونایروس، نقش اساسی مدیریت کلاسی ارتقا کیفیت باید مورد قرار گیرد این پژوهش هدف تدوین مدل رابطه علّی هدفمند کلاس وضعیت روان‌شناختی پیامدهای تحصیلی دانشجویان انجام شد. روش: روش بر حسب هدف، کاربردی ماهیت از نوع توصیفی - همبستگی بود. جامعه آماری مشتمل انواع مقاطع دانشگاه­ های دولتی پیام نور شهر تهران نمونه اساس جدول مورگان ب...

We developed a multiplex enzyme-based electrochemical genosensor for sequence-specific detection of multiplex linear-after-the-exponential-PCR amplicons that targeted toxigenic Vibrio cholerae O1 and O139 using novel screen-printed gold electrode bisensors.