Leaf spot symptoms were spotted in two olive orchards Istria and Kvarner Gulf, Croatia. Fungal species from three representative isolates (P13 LECIII, R18 BI, JA20 NP) have been morphologically characterized based on the colony conidial characteristics. Several techniques performed for inducing sporulation of NP isolate. Only PDA + banana medium was successful. PCR conducted ITS, TUB, EF1α gene...