A combined approach based on isothermal titration calorimetry (ITC), fluorescence resonance energy transfer (FRET) experiments, circular dichroism spectroscopy (CD), atomic force microscopy (AFM) dynamic force spectroscopy (DFS), and surface plasmon resonance (SPR) was applied to elucidate the mechanism of protein-DNA complex formation and the impact of protein dimerization of the DNA-binding d...

Demam Berdarah Dengue (DBD) merupakan infeksi akibat virus dengue yang ditularkan oleh nyamuk Aedes spp. Kasus DBD di Sumatera Selatan menunjukan peningkatan signifikan dari tahun ke tahun. Faktor penyebab yaitu rendahnya pengetahuan masyarakat mengenai pencegahan dan pemberatasan penyakit DBD. Tujuan kegiatan ini untuk melakukan penyuluhan kesehatan sebagai upaya mengurangi kepadatan dengan pe...

DNA repair takes place in the context of chromatin. Previous studies showed that histones impair base excision repair (BER) of modified bases at both the excision and synthesis steps. We examined BER of uracil in a glucocorticoid response element (GRE) complexed with the glucocorticoid receptor DNA binding domain (GR-DBD). Five substrates were designed, each containing a unique C-->U substituti...

Aerosol particle charging is involved in many scientific and industrial applications and especially in measurements based on electrostatic classification. Non-thermal Atmospheric Pressure Electrical Discharges are an efficient way to charge particles. We report here the charge distribution of aerosol at the exit of a Dielectric Barrier Discharges (DBD) and discuss the potential applications. Th...

OBJECTIVES To describe the signalment, presence of systemic and/or ocular comorbidities, times to detected healing and probabilities of recurrence after diamond burr debridement (DBD) of eyes with presumed corneal calcareous degeneration and secondary ulceration and/or ocular pain. ANIMALS STUDIED Twenty-six dogs with 42 eyes affected, 34 eyes treated with DBD. METHODS A case series was con...

BACKGROUND NR2E3 (PNR) is an orphan nuclear receptor essential for proper photoreceptor determination and differentiation. In humans, mutations in NR2E3 have been associated with the recessively inherited enhanced short wavelength sensitive (S-) cone syndrome (ESCS) and, more recently, with autosomal dominant retinitis pigmentosa (adRP). NR2E3 acts as a suppressor of the cone generation program...

Pendampingan dan penyuluhan kesehatan tentang DBD merupakan salah satu cara yang digunakan untuk menambah pengetahuan seseorang dengan tujuan mengubah atau mempengaruhi perilaku manusia dalam pencegahan DBD. Tujuan Penelitian Untuk mengetahui pengaruh pendampingan demam berdarah dengue terhadap sikap kepala keluarga. Metode penelitian  menggunakan pra eksperimen bentuk one group pretest and po...

Deficits in emotional reactivity are frequently reported in Disruptive Behavior Disorders (DBDs). A deficit in prosocial emotions, namely the callous unemotional traits (CU), may be a mediator of emotional reactivity. Our aim is to investigate subjective emotional reactivity towards visual stimuli with different affective valence in youths with DBDs and healthy controls. The clinical sample inc...

The latency-associated nuclear antigen (LANA) of Kaposi's sarcoma-associated herpesvirus functions as an origin-binding protein (OBP) and transcriptional regulator. LANA binds the terminal repeats via the C-terminal DNA-binding domain (DBD) to support latent DNA replication. To date, the structure of LANA has not been solved. Sequence alignments among OBPs of gammaherpesviruses have revealed th...

FOXA2, a member of the forkhead family of transcription factors, plays essential roles in liver development and bile acid homeostasis. In this study, we report a 2.8 Å co-crystal structure of the FOXA2 DNA-binding domain (FOXA2-DBD) bound to a DNA duplex containing a forkhead consensus binding site (GTAAACA). The FOXA2-DBD adopts the canonical winged-helix fold, with helix H3 and wing 1 regions...