To investigate the role of ROS in the helicobacter pylori (Hp) induced mtDNA mutations, AGS cells were treated by extracts of Hp11638 or Hp11638M. The ROS levels, cytochrome C reductions, and intracellular ATP levels were measured. The coding region and the D-Loop region were amplified and sequenced. Results showed the ROS levels, cytochrome C reduction and mtDNA mutations were markedly increas...

calomyscus mystax is endemic to great balkhan mountains in southwestern turkmenistan. however there have been some inaccurate reports of existing this species in koprtdagh mts. and northern iran, but these were considered as calomyscus elburzensis and calomyscus grandis. phylogenetic analyses on cytb gene clustered samples from kopetdagh mts. in northeastern iran with one specimen of c. mystax ...

the barn owl, tyto alba (scopoli, 1769), occurs worldwide and shows a considerable amount of morphological and geographical variation, leading to the recognition of many subspecies around the world. yet comprehensive study on this species needs to be done. data from mitochondrial gene (cytb) with 620bp length is analyzed for 30 individuals around the world. maximum likelihood (ml), maximum pars...

The genetic diversity of Taenioides cirratus was investigated on the basis mitochondrial cytochrome c oxidase subunit I (COI) and b (Cytb) gene sequences. A total 159 specimens collected from Chaohu Lake (CL), Nansihu (NL), Taihu (TL), Pearl River (PR), Nandu (NR) were sequenced. length sequence 2485 bp with 412 polymorphic sites. 73 haplotypes identified, Hap1 being most widely distributed. PR...

Cryptic and polymorphic species can complicate traditional taxonomic research and both of these concerns are common in fig wasp communities. Species identification is very difficult, despite great effort and the ecological importance of fig wasps. Herein, we try to identify all chalcidoid wasp species hosted by one species of fig, using both morphological and molecular methods. We compare the e...

We describe the case of a woman in whom combination of a mitochondrial (MT-CYB) and a nuclear (SDHB) mutation was associated with clinical and metabolic features suggestive of a mitochondrial disorder. The mutations impaired overall energy metabolism in the patient's muscle and fibroblasts and increased cellular susceptibility to oxidative stress. To clarify the contribution of each mutation to...

An ethidium bromide-induced stopper mutant of Neurospora crassa is characterized at the molecular level. The mutant has two populations of mitochondrial DNA: a defective predominant mutant molecule and a basal level of the wild-type molecule. The aberrant DNA resulted after a 25-kbp deletion from the wild-type mitochondrial chromosome, which included major genes such as cytb, co1 and oli2. The ...

A recent molecular phylogeny of the Arid clade of the genus Hemidactylus revealed that the recently described H. saba and two unnamed Hemidactylus species from Sinai, Saudi Arabia and Yemen form a well-supported monophyletic group within the Arabian radiation of the genus. The name 'Hemidactylus saba species group' is suggested for this clade. According to the results of morphological compariso...

Relationships among the species of Northern Hemisphere smelts (family Osmeridae) have long been debated in the fish systematics literature. Eight independent studies based on morphological characters failed to reach any consensus on osmerid interrelationships. We reconstruct the osmerid phylogeny based on DNA sequence data from three mitochondrial (cytb, 16S, 12S) and three nuclear (ITS2, S71, ...

The lesser Japanese mole, Mogera imaizumii, recognized by Motokawa and Abe (1996), occurs in eastern Honshu, western Honshu and Shikoku. Mitochondrial and nuclear DNA were analyzed for mole samples from eastern Honshu to elucidate intraspecific differentiation. Analyses of sequences of the mitochondrial cytochrome b gene (Cytb) and of a restriction fragment length polymorphism of the nuclear 28...