Membranes of human erythrocytes were prepared by stepwise osmotic hemolysis in Ca2+-free solutions. Examination with the electron microscope after negative staining showed some short, conelike protuberances on the surface of about 20 percent of the ghosts, while 80 percent were round, intact spheres. After Ca2+ treatment, all membranes were round and intact. After exposure to ethylenediaminetet...

The photosynthetic assimilates in leaves of Perilla crispa attached to the plant were labeled by treating the leaves with (14)CO(2). When subsequently detached, these leaves exuded a negligible amount of radioactivity from the cut petiole into water. Ethylenediaminetetraacetate (EDTA), citric acid, and ethyleneglycol-bis (beta-aminoethyl ether) N,N'-tetraacetate greatly increased exudation of l...

Thiol-disulfide isomerization in thrombospondin may affect the function of this adhesive protein. Two assays were developed to analyze the determinants of thiol-disulfide exchange and to correlate this exchange with thrombospondin conformation. (1) A competitive immunoassay for the EDTA-conformation of thrombospondin was developed with monoclonal antibody D4.6. (2) The free thiol(s) in thrombos...

Ethylenediaminetetraacetic acid (EDTA) was developed by Franz Munz inGermanyduring the 1930s as an alternative to citric acid. About the same time, Frederick Bersworth synthesized EDTA in the United States using a different technique. He obtained aUSpatent for this technique in1945. EDTAwas approvedby the US Food and Drug Administration (FDA) as a food additive in 1947. Since the early 1950s, E...

Brucellosis is a zoonotic bacterial disease with worldwide distribution. A cross-sectional study was conducted to determine the serological prevalence of brucellosis in horses within Kano Metropolis. total 328 serum samples were collected for study. Modified Rose Bengal Plate Test (mRBPT) on all while Serum Agglutination EDTA (SAT-EDTA) mRBPT-positive samples. The metropolis found be 24.09 % (7...

To the Editor: Increased plasma homocysteine is regarded as a risk factor for arterial and venous occlusive disease. Sample collection ideally requires use of cooled EDTA containers and immediate centrifugation of the blood (1). These conditions may be hard to maintain in a clinic and are not possible in the many medical practices that lack a centrifuge. We tested whether analyzing uncentrifuge...

Accepted I September 1994 Abstract Objectives-To validate a provocative chelation test with 2,3-dimercaptosuccinic acid (DMSA) by direct comparison with the standard ethylene diamine tetraacetic acid (EDTA) test in the same subjects; and to compare and contrast the predictors of lead excretion after DMSA with those after EDTA. A metal chelating agent given orally, DMSA may mobilise and enhance ...

[58Co]EDTA, [51Cr]RBC and [125I]albumin spaces in the whole body and 28 tissue samples were examined at timed intervals over 16 h in rainbow trout Oncorhynchus mykiss. [58Co]EDTA space (which approximates extracellular fluid volume; ECF) in fins, skin, gallbladder and eye are reported for the first time. After a 16 h equilibration, ECF volume was large (376-726 microl g-1 wet tissue mass) in ki...

This work presents a novel collection method for gunshot residues (GSR) using a sampling procedure based on ethylenediaminetetraacetic acid (EDTA) solution as a complexing agent on moistened swabs. Detection was via a sector-field inductively coupled plasma mass spectrometry (HRICP-MS). The proposed collection and analytical method allowed detection of antimony (Sb), barium (Ba) and lead (Pb) a...