This study was concerned with the effects of NaCl administered in vivo or added in vitro to isolated nuclei on [(3)H]tryptophan binding to rat hepatic nuclei assayed in vitro. Hypertonic (10.7%) NaCl administered in vivo to rats caused at 10 min a marked decrease in in vitro binding (total and specific) of [(3)H]tryptophan to hepatic nuclei. In vitro incubation of isolated hepatic nuclei, but n...

The present studies investigated the influence of presystemic signals on the control of thirst, salt appetite, and vasopressin (VP) secretion in rats during nonhypotensive hypovolemia. Rats were injected with 30% polyethylene glycol (PEG) solution, deprived of food and water overnight, and then allowed to drink water, 0.15 M NaCl, or 0.30 M NaCl. The PEG treatment, which produced 30-40% plasma ...

BACKGROUND Algal cells produce neutral lipid when stressed and this can be used to generate biodiesel. OBJECTIVE Salt stressed cells of the model microalgal species Chlamydomonas reinhardtii were tested for their suitability to produce lipid for biodiesel. METHODS The starchless mutant of C. reinhardtii (CC-4325) was subjected to salt stress (0.1, 0.2 and 0.3 M NaCl) and transesterification...

A 60to 125-fold purification of nuclear extracts from rat liver triiodothyronine receptor was achieved by dialysis of 0.4 M NaCl nuclear extracts followed by chromatography on DEAE-Sephadex. Extract was labeled either by injecting tracer [““Iltriiodothyronine into the animal or by direct addition of the tracer in. vitro prior to chromatography. Elution of the column with a linear NaCl gradient ...

During a single cycle of multiplication of type 4 adenovirus in HeLa cells an average 2-fold increase in total DNA occurred over that measured in uninfected cells. Of the total DNA from infected cells about 23 per cent was extractable into 0.15 M NaCl, a quantity approximately four and a half times greater than that of the DNA obtained from normal cells in 0.15 M NaCl. Ninety to 99 per cent of ...

Unexpectedly high culturable diversity of moderately and extremely halophilic obligately chemolithoautotrophic sulfur-oxidizing bacteria (SOB) was discovered in the sediments of various hypersaline habitats, including chloride-sulfate lakes in Mongolia, Russia and Ukraine, a sea saltern in Slovenia and a deep-sea salt brine from the Mediterranean. Six different groups of halophilic SOB, includi...

Amiloride, a sodium channel blocker, is known to suppress NaCl responses of the chorda tympani (CT) nerve in various mammalian species. In mice, the NaCl suppressing effect of amiloride is reported to differ among strains. In C57BL mice, amiloride inhibits NaCl responses to about 50% of control, whereas no such clear suppression was evident in prior studies with 129 mice. However, evidence from...

27 28 Regulation of the putative amiloride and benzamil (Bz)-insensitive TRPV1t salt 29 taste receptor by phosphatidylinositol 4, 5-bisphosphate (PIP2) was investigated by 30 monitoring chorda tympani (CT) taste nerve responses to 0.1M NaCl solutions 31 containing Bz (5x10 M; a specific ENaC blocker) and resiniferatoxin (RTX; 0-10x10 32 M; a specific TRPV1 agonist) in Sprague-Dawley rats and in...

A minimalist Go-model, with no energetic frustration in the native conformation, has been shown to describe accurately the folding pathway of the beta-trefoil protein, interleukin-1beta (IL-1beta). While it appears that these models successfully model transition states and intermediates between the unfolded and native ensembles, it is unclear how accurately they capture smaller, yet biologicall...

Background: Salinity is a major environmental limiting factor, which affect agricultural production. The two Manilkara seedlings (M. roxburghiana and M. zapota) with high economic importance, could not adapt well to higher soil salinity and little is known about their proteomic mechanisms. Objectives: The mechanisms responsible ...