In this study, we have identified a novel Nedd4-like ubiquitin ligase, BUL1, as the host factor involved in budding of type D retrovirus Mason-Pfizer monkey virus (M-PMV). Overexpression of BUL1 enhanced virus particle release, while a BUL1 mutant in which a W to G substitution was introduced into a WW domain, W791G, lost the ability to bind to the viral Gag protein and abolished its ability to...

The budding reactions of a number of enveloped viruses use the cellular machinery involved in the formation of the luminal vesicles of endosomal multivesicular bodies (MVB). Budding of these viruses is dependent on the presence of specific late-domain motifs in membrane-associated viral proteins. Such budding reactions usually involve ubiquitin and are blocked by expression of an ATPase-deficie...

UNLABELLED It has been reported that poorly-differentiated clusters of cancer cells at the invasive front, namely "tumour budding", may reflect malignancy of colorectal cancer. The aim of the present study was to evaluate the activity of cathepsin D and B in tumours and in normal mucosa from pT3 and G2 colorectal cancers, and to analyse their association with tumour budding at the invasion fron...

background: over the last 10 years, the evidential practice has been developing in kazakhstan. the clinical protocols of diagnostics and treatment were implemented in the country since 2007. we analyzed the normal birth practice in kazakhstan. materials and methods: obstetricians and midwives were subject to the questionnaire survey in four large maternity hospitals of almaty. results: totally,...

Background: Colorectal adenocarcinoma (CA) is one of the most common malignancies. Tumor budding (TB) status associated with poor prognosis in patients. Prognosis influenced by clinicopathological profile. This study aims to determine relationship between profile TB CA at Dr. Cipto Mangunkusumo General National Hospital.Method: A cross-sectional retrospective analytic using secondary data form ...

Background and Purpose: Formation of pseudohyphae is considered a virulence factor in Candida species. Generally, Candida glabrata grows as budding yeast cells; however, reports illustrated that C. glabrata could form pseudohyphal cells in response to some stimuli. In this study, we provided insight into the ability of C. glabrata in forming pseudohyphal cells under different levels of carbon d...