Mitotic cell division involves the equal segregation of all chromosomes during anaphase. The presence of ribosomal DNA (rDNA) repeats on the right arm of chromosome XII makes it the longest in the budding yeast genome. Previously, we identified a stage during yeast anaphase when rDNA is stretched across the mother and daughter cells. Here, we show that resolution of sister rDNAs is achieved by ...

St. John's wort is a widely used medicinal plant. The quality of herbal drug, which is in most of the cases collected from nature, varies. Therefore, the aim of the present study was detailed chemical characterization of Hypericum perforatum subsp. perforatum samples collected in close time intervals during flowering and fruitification with the purpose to state the phenological stage characteri...

The study evaluated the response of alfalfa (Medicago sativa L) to arbuscular mycorrhizal fungi (AM) species Glomus intraradices and Sinorhizobium meliloti, strain 1021 regarding the dry biomass accumulation, mycorrhizal fungi colonization, nodulation and nitrogen fixation activity. Alfalfa plants were grown in a glasshouse until the flowering stage (58 days), in 4 kg plastic pots using leached...

Adult medusae of Carukia barnesi were collected near Double Island, North Queensland Australia. From 73 specimens, 8 males and 15 females spawned under laboratory conditions. These gametes were artificially mixed which resulted in fertilized eggs. Post fertilization, most eggs developed to an encapsulated planula stage and then paused for between six days and six months prior to hatching as cil...

The rate of eukaryotic cell growth is tightly controlled for proper progression through each cell cycle stage and is important for cell size homeostasis. It was previously shown that cell growth is inhibited during mitosis when cells are preparing for division. However, the mechanism for growth arrest at this stage is unknown. Here we demonstrate that exocytosis of a select group of cargoes was...

In early embryogenesis, the posteroventral foregut endoderm gives rise to the budding endodermal organs including the liver, ventral pancreas and gallbladder during early somitogenesis. Despite the detailed fate maps of the liver and pancreatic progenitors in the mouse foregut endoderm, the exact location of the gallbladder progenitors remains unclear. In this study, we performed a DiI fate-map...

Background: Colorectal adenocarcinoma (CA) is one of the most common malignancies. Tumor budding (TB) status associated with poor prognosis in patients. Prognosis influenced by clinicopathological profile. This study aims to determine relationship between profile TB CA at Dr. Cipto Mangunkusumo General National Hospital.Method: A cross-sectional retrospective analytic using secondary data form ...

Ebola virus budding is mediated by two proline-rich motifs, PPxY and PTAP, within the viral matrix protein VP40. We have previously shown that a Nedd4-like protein BUL1, but not Nedd4, positively regulates budding of type D retrovirus Mason-Pfizer monkey virus (J. Yasuda, E. Hunter, M. Nakao, and H. Shida, EMBO Rep. 3:636-640, 2002). Here, we report that the cellular E3 ubiquitin ligase Nedd4 r...

The aim of this study is trying to describe more details of superior mesenteric artery margin in pancreaticoduodenectomy for pancreatic ductal adenocarcinoma, to evaluate biological and prognostic implications of tumor budding in this margin, and to provide more evidence for evaluation of R0 surgery in pancreaticoduodenectomy. 46 patients in 5-years period are included in this study. Immunochem...