The effect of disease-causing missense mutations on protein folding is difficult to evaluate. To understand this relationship, we developed the unfolding mutation screen (UMS) for in silico evaluation of the severity of genetic perturbations at the atomic level of protein structure. The program takes into account the protein-unfolding curve and generates propensities using calculated free energ...

1. Knoshi J, lida Y, Kousaka T, et al. Effect of antithyroxin autoantibodies on radioimmunoassay of free thyroxin in serum. Clin Chem 28, 1389-1391 (1982). Case report. 2. Mullinger RN, Walker G. Free thyroxin in thyroid disease. Clin Chem 28, 13941395 (1982). Letter. 3. Bhagat CI, Garcia-Webb P, Watson F, Beilby JP. Interference in radioimmunoassay of total serum thyroxin and free thyroxin due...

BACKGROUND Dyslipidemia after kidney transplantation is a frequent finding and is multifactorial. Immunosuppressive agents such as cyclosporine (CsA) can cause hypercholesterolemia. OBJECTIVES As there were few reports with conflicting evidence on whether CsA related dyslipidemia is dose related and that CsA monitoring assays (trough level, C0, or two hour post dose level, C2) is a better pre...

The incidence and prognostic impact of nephropathy related to Waldenström macroglobulinaemia (WM) is currently unknown. We performed a retrospective study to assess biopsy-confirmed WM-related nephropathy in a cohort of 1391 WM patients seen at a single academic institution. A total of 44 cases were identified, the estimated cumulative incidence was 5·1% at 15 years. There was a wide variation ...

OBJECTIVE In this study, we aimed to detect the incidences of ototoxicity in patients with hemoglobinopathies taking deferoxamine (DFO), deferiprone, and deferasirox using the National Cancer Institute (NCI) Common Terminology Criteria for Adverse Events (CTCAE) scale to obtain more objective data. MATERIALS AND METHODS Fifty-five transfusion-dependent patients were evaluated in this study. T...

We report here a procedure which results in the purification of an extracellular protease (designated Ps-1) from Pseudomonas aeruginosa. This enzyme cleaves fibrinogen so that the modified molecules form microcrystals and large single crystals. Precise knowledge of the Ps-1 cleavage sites is essential for the interpretation of the structural information provided by these crystals (Weisel, J. W....

8159.2011.03112.x. [11] Sakellaridis T, Argiriou M, Panagiotakopoulos V, Charitos C. Bilateral sternobronchial fistula after coronary surgery—are retained epicardial pacing wires responsible. J Cardiothorac Surg 2009;4:26. [12] Horng GS, Ashley E, Balsam L, Reitz B, Zamanian RT. Progressive dyspnoea after CABG: complication of retained epicardial pacing wires. Ann Thorac Surg 2008;86:1352–4. [1...

1510 plasma cholesterol estimations were made in 1391 children admitted to hospital as part of a biochemical profile. Babies under 1 year and children known to have familial hyperlipoproteinaemia were excluded. The mean concentration was 4-28 mmol/l +/- 1-04 (1 SD) (165-3 mg/100 ml +/- 38-6), and levels exceeded 5-93 mmol/l (229 mg/100 ml) in 68 children. Repeat estimations on 55 of these child...

Abstract Objective: To identify regional cerebral blood flow (rCBF) differences among children with varying Attention-deficit/hyperactivity disorder (ADHD) subtypes. The participants were part of an archival de-identified database that received SPECT scans at baseline. sample (N= 3222) consisted ADHD classifications asymptomatic (N=773), combined type (N=1157), hyperactive (N=25), inattentive (...