نتایج جستجو برای: 16s by pcr

تعداد نتایج: 7109237  

Journal: :Applied and environmental microbiology 1996
M T Suzuki S J Giovannoni

The PCR is used widely for the study of rRNA genes amplified from mixed microbial populations. These studies resemble quantitative applications of PCR in that the templates are mixtures of homologs and the relative abundance of amplicons is thought to provide some measure of the gene ratios in the starting mixture. Although such studies have established the presence of novel rRNA genes in many ...

Journal: :Microbiology 1996
F A Rainey N L Ward-Rainey P H Janssen H Hippe E Stackebrandt

Sequence analysis of the cloned 16S rRNA genes of Clostridium paradoxum DSM 7308T revealed the presence of 15 different sequences in variable region I (Escherichia coli position 73-97) of the 16S rRNA. The majority of the cloned genes contained intervening sequences (IVSs), which varied in length from 120-131 nt, and were present in the DNA obtained from single colonies of C. paradoxum. The abs...

Journal: :Veterinary microbiology 2008
William R Hare Phillip G Hoyt Christina Hohn James A Higgins

Bovine keratoconjunctivitis (BKC), colloquially referred to as 'pinkeye', is a disease affecting cattle worldwide; it costs cattle producers millions of dollars in economic loss annually. While Moraxella spp. are the primary etiologic agent of pinkeye, surveys of flora from the conjunctivae of livestock from around the world have indicated that a variety of bacterial commensals occupy this nich...

Fereydoon Padasht Dehkaei Gholam Hosein Shahidi Bonjar Meysam Soltani Nejad,

Bipolaris oryzae causes rice brown spot disease which reduces rice yield with substantial global impact. Streptomycetes isolated from rice fields of Guilan Province, Iran, showed antifungal activity against the tested pathogen. From five active Streptomyces spp. isolates, isolate G showed high antagonistic activity. To determine its taxonomical identity, its colonies character...

ژورنال: :علوم و فنون دریایی 0
زینب احمدی دانشگاه علوم و فنون دریایی خرمشهر حسین ذوالقرنین دانشگاه علوم و فنون دریایی خرمشهر_عضو هیأت علمی بیتا ارچنگی دانشگاه علو.م و فنون دریایی خرمشهر_عضو هیأت علمی ابراهیم رجب زاده دانشگاه علوم و فنون دریایی خرمشهر_عضو هیأت علمی

16s rrna یکی از اجزای بخش بزرگ ریبوزومی می باشد که بوسیله ی ژنوم میتوکندریایی کد می شود و به طور گسترده ای در مطالعات فیلوژنتیکی مربوط به گونه های نزدیک و به خصوص گونه های مربوط به crassostrea مورد استفاده قرار می گیرد. پلی مورفیسم توالی نوکلئوتیدی مربوط به ژن 16s rrna ژنوم میتوکندریایی اویستر خلیج فارس (crassostrea sp.)، در 30 نمونه اویستر جمع آوری شده از بندر امام خمینی مورد بررسی قرار گرفت. ...

Journal: :Genetics and molecular research : GMR 2004
Dirce Maria Carraro Anamaria Aranha Camargo Anna Christina Matos Salim Luiz Gonzaga Gisele Cavalcante da Costa Ana Tereza Ribeiro de Vasconcelos Andrew J G Simpson

In the finishing phase of the Chromobacterium violaceum genome project, the shotgun sequences were assembled into 57 contigs that were then organized into 19 scaffolds, using the information from shotgun and cosmid clones. Among the 38 ends resulting from the 19 scaffolds, 10 ended with sequences corresponding to rRNA genes (seven ended with the 5S rRNA gene and three ended with the 16S rRNA ge...

Journal: :Environmental microbiology 2014
Ramiro Logares Shinichi Sunagawa Guillem Salazar Francisco M Cornejo-Castillo Isabel Ferrera Hugo Sarmento Pascal Hingamp Hiroyuki Ogata Colomban de Vargas Gipsi Lima-Mendez Jeroen Raes Julie Poulain Olivier Jaillon Patrick Wincker Stefanie Kandels-Lewis Eric Karsenti Peer Bork Silvia G Acinas

Sequencing of 16S rDNA polymerase chain reaction (PCR) amplicons is the most common approach for investigating environmental prokaryotic diversity, despite the known biases introduced during PCR. Here we show that 16S rDNA fragments derived from Illumina-sequenced environmental metagenomes (mi tags) are a powerful alternative to 16S rDNA amplicons for investigating the taxonomic diversity and s...

Journal: :international journal of advanced biological and biomedical research 2014
meysam soltani nejad gholam hosein shahidi bonjar fereydoon padasht dehkaei

bipolaris oryzae causes rice brown spot disease which reduces rice yield with substantial global impact. streptomycetes isolated from rice fields of guilan province, iran, showed antifungal activity against the tested pathogen. from five active streptomyces spp. isolates, isolate g showed high antagonistic activity. to determine its taxonomical identity, its colonies characterized morphological...

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