. cobalamin . + homocysteine p H4PteGlu + methionine. In the absence of cobalamin the reaction is impaired, the methyl group remains on tetrahydrofolate (H4PteGlu), and a critical lack of free tetrahydrofolate develops. As a result, single-carbon units such as formate and methylene cannot be taken up because of lack of a folate acceptor and synthesis of purines requiring formate, and that of th...

Amethopterin in toxic amounts, whether given in a single dose or in repeated daily doses, produced no observable inhibition of the nicotinamide-induced synthesis of diphosphopyridine nucleotide in mouse liver in vivo. Likewise, no inhibition was de tected of the incorporation of formate-C14 into the total liver acid-soluble adenine. However, administration of amethopterin sufficient to produce ...

GTP cyclohydrolase I catalyzes a mechanistically complex ring expansion affording dihydroneopterin triphosphate and formate from GTP. Single turnover quenched flow experiments were performed with the recombinant enzyme from Escherichia coli. The consumption of GTP and the formation of 5-formylamino-6-ribosylamino-2-amino-4(3H)-pyrimidinone triphosphate, formate, and dihydroneopterin triphosphat...

Due to concerns over the resistance development existing pesticides, chemical poisoning among farmers, and residue on crops, sprung up a growing need develop new pest control strategies for utilization in protected houses Korea. A series of experiments tested technology using fumigant, ethyl formate, crops houses. It was revealed that glasshouse inadequate fumigation system fumigant since forma...

Mixtures of yttrium acetate hydrate (Y(O2CCH3)3?4H2O) and aluminum formate hydrate (Al(O2CH)3?3H2O in H2O) or yttrium isobutyrate (Y[O2CCH(CH3)2]3) and aluminum isobutyrate (Al[O2CCH(CH3)2]3) in tetrahydrofuran were used as precursors to process yttrium aluminum garnet (YAG, Al5Y3O12) fibers. The pyrolytic decomposition patterns of Al(O2CH)3?3H2O, Y(O2CCH3)3?4H2O, and a [3Y(O2CCH3)3?4H2O/5Al(O2...

Hydrogen fuel is renewable, efficient and clean, and fermentative bacteria hold great promise for its generation. Here we use the isogenic Escherichia coli K-12 KEIO library to rapidly construct multiple, precise deletions in the E. coli genome to direct the metabolic flux towards hydrogen production. Escherichia coli has three active hydrogenases, and the genes involved in the regulation of th...

Pyruvate is located at a metabolic junction of assimilatory and dissimilatory pathways and represents a switch point between respiratory and fermentative metabolism. In Escherichia coli, the pyruvate dehydrogenase complex (PDHC) and pyruvate formate-lyase are considered the primary routes of pyruvate conversion to acetyl-CoA for aerobic respiration and anaerobic fermentation, respectively. Duri...

Several recent, reports from this laboratory have implicated vitamin E in the metabolism of the nucleic acids. It was shown that vitamin E-deficient rabbits excrete extra quantities of allantoin (1) and that the livers from such animals exhibit greatly increased xanthine oxidase activities (2). Finally it haa been shown that vitamin E deprivation in the rat, leads to an increased incorporation ...

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