Bupivacaine-induced regeneration was studied in the rat soleus muscle in the presence or absence of innervation, in the presence of tetrodotoxin (TTX)-induced block of nerve impulse conduction, and/or in the presence of vinblastine-induced block of nerve axoplasmic flow. Part of experiments were carried out on tenotomized muscles. Regenerated muscles were analysed for myosin heavy chain (MHC) c...

We report the initial characterization of an N-terminal oligopeptide '2A-like' sequence that is able to function both as a signal sequence and as a translational recoding element. Owing to this translational recoding activity, two forms of nascent polypeptide are synthesized: (i) when 2A-mediated translational recoding has not occurred: the nascent polypeptide is fused to the 2A-like N-terminal...

The mutation of Ala-i 17 to Val conferred dehydroepiandrosterone (DHEA) hydroxylase activity on cytochrome P-450 2a-4, with the production of both 2aand 7ahydroxyDHEA at similar rates. P-450 2a-5 which has Val at position 117, acquired high DHEA hydroxylase activity by mutation of Phe-209. Mutant F209L of P-450 2a-5 exhibited strong regiospecificity at the 2-position of the DHEA molecule with t...

Rates of autophagy, the mechanism responsible for lysosomal clearance of cellular components, decrease with age. We have previously described an age-related decline in chaperone-mediated autophagy (CMA), a selective form of autophagy, by which particular cytosolic proteins are delivered to lysosomes after binding to the lysosome-associated membrane protein type 2A (LAMP-2A), a receptor for this...

BACKGROUND A major pathological hallmark of AD is the deposition of insoluble extracellular beta-amyloid (Abeta) plaques. There are compelling data suggesting that Abeta aggregation is catalysed by reaction with the metals zinc and copper. METHODOLOGY/PRINCIPAL FINDINGS We now report that the major human-expressed metallothionein (MT) subtype, MT-2A, is capable of preventing the in vitro copp...

conclusions treatment with peg ifn α achieved a sustained negativity of hdv rna in about one third of patients. duration of peg ifn α therapy might be prolonged to at least 24 months or more to prevent the occurrence of hepatitis b virus (hbv) relapse encountered six months after completion of the treatment. patients and methods the sample size was based on available patients potentially to be ...

PURPOSE To determine whether recombinant human interferon alpha-2a (IFN alpha-2a) would diffuse into the choroid in significant amounts from a retrobulbar depot. METHODS One million international units of IFN alpha-2a were injected into the retrobulbar space of the eyes of 17 rabbits, and choroidal and serum concentrations were measured at 1, 2, 4, 8, 12, and 24 hours. The same dose of IFN al...

(-)-Epigallocatechin-3-gallate (EGCG) is a well-known chemoprevention factor. Recent studies have revealed that EGCG triggers cancer cells undergoing apoptosis through p53-dependent pathway. How EGCG activates p53-dependent apoptosis is not fully understood. In the present study using JB6 cell as a model system, we have shown that EGCG can negatively regulate protein serine/threonine phosphatas...

barium salts of dibenzylidene cystines 8a-c were obtained from the reaction of lcystine with benzaldehyde or its derivatives in the presence of barium hydroxide. their reduction with zinc and hydrochloric acid in methanol yielded the narylmethylcysteines ?9a-c?. these can, in turn, be converted by esterification into the methyl esters ?10 a-c. ?reaction of n, n'-carbonyl diimidazole (im co...