In the net reaction catalyzed by homogentisate oxidase of rat liver, two atoms of osygen are added to the double bond between carbon atoms 1 and 2 of homogentisate (2, 3) resulting in the formation of maleylacetoacetate (Fig. 1). The enzyme is located in the soluble phase of the liver cell; its activity is totally independent of the hydrogen and electron transport system. Its properties and mod...

Water demand is increasing because of demographic and urban development in the last decades. Crude oil an essential energy resource for many anthropogenic activities. However, it associated with generation environmental pollution. In present work, a native microbial consortium was used to study hydrocarbon biodegradation crude its potential use remove organic pollutants wastewater. The kinetic ...

Emerging evidence supports an important role for the heme oxygenase system (HO-1) in the maintenance of a healthy pregnancy, especially during pathological challenge. HO-1 is widely accepted to be a highly sensitive and reliable marker of oxidative stress. Hence the present study was planned to analyse heme oxygenase-1 and lipid profile in maternal and cord blood venous samples of normal pregna...

The two xylE genes for catechol 2,3-oxygenase, encoded by TOL plasmid pWW53, carry a common SalI restriction site within the reading frame. Each gene was cut at the SalI site and the 5' end of each gene spliced to the 3' end of the other to form hybrid genes, from both of which catalytically active catechol 2,3-oxygenase activities were expressed. The kinetic parameters were determined for the ...

Indoleamine-2,3-dioxygenase-1 (IDO1) is a critical immunoregulatory enzyme responsible for the metabolism of tryptophan during inflammation and disease. Based upon a pyranonaphthoquinone framework, the first examples of indoleamine-2,3-dioxygenase-1 (IDO1) inhibitors containing a carborane cage are reported. The novel closo-1,2-carboranyl-N-pyranonaphthoquinone derivatives display low μM bindin...

One of the three components of the naphthalene dioxygenase occurring in induced cells of Pseudomonas sp. strain NCIB 9816 has been purified to homogeneity. The protein contained 2 g-atoms each of iron and acid-labile sulfur and had an apparent molecular weight of 13,600. The evidence indicates that it is a ferredoxin-type protein that functions as an intermediate electron transfer protein in na...

Gamma interferon (IFN-gamma) affects tryptophan metabolism by mediating the expression of indoleamine 2,3-dioxygenase and tryptophanyl-tRNA synthetase. In the present study, we investigated the role of indoleamine 2,3-dioxygenase-mediated tryptophan depletion in the induction of tryptophanyl-tRNA synthetase by IFN-gamma. The addition of excess tryptophan to the culture medium did not affect the...

A 4,103-bp long DNA fragment containing the structural gene of a gentisate 1,2-dioxygenase (EC 1.13.11.4), gtdA, from Sphingomonas sp. strain RW5 was cloned and sequenced. The gtdA gene encodes a 350-amino-acid polypeptide with a predicted size of 38.85 kDa. Comparison of the gtdA gene product with protein sequences in databases, including those of intradiol or extradiol ring-cleaving dioxygena...