Dideoxy chain-termination DNA sequencing was used to determine the specific DNA base changes induced after in vivo exposure of Escherichia coli to N-methyl-N-nitrosourea (MNU) and N-ethyl-N-nitrosourea (ENU) using the xanthine guanine phosphoribosyltransferase (gpt) gene as the genetic target. The resultant mutation spectra were compared with the levels of O6-alkylguanine and O4-alkylthymidine ...

Animal cells and cell lines, such as HEK-293 cells, are commonly cultured at 37°C. These cells are often used to express recombinant proteins. Having a higher expression level or a higher protein yield is generally desirable. As we demonstrate in this study, dropping culture temperature to 33°C, but not lower, 24 hours after transient transfection in HEK-293S cells will give rise to ~1.5-fold h...

Despite aggressive treatment with radiation and chemotherapy, recurrence of glioblastoma multiforme (GBM) is inevitable. The objective of this study was to show that the blood-brain barrier (BBB), through a combination of tight junctions and active efflux transporters in the brain microvasculature, can significantly restrict delivery of molecularly targeted agents to invasive glioma cells. Tran...

Hawaii 167. Our observations confirm the hypothesis that Hawaii 167 contains a massive quantity of dust, the optical depth of which is sufficient to extinguish completely our ultraviolet view of a central, buried quasar. The submillimetre luminosity and associated dust mass of Hawaii 167 are similar to those of the ultraluminous class of infrared galaxies, supporting the existence of an evoluti...

Orthopedic surgery is common in pediatric patients because of the wide variety of bone and muscle injury or disease during childhood.These entities range from simple fractures in healthy children to complex spinal fusions in children with debilitating neuromuscular disorders. Anesthetic considerations for children with these specific disorders are covered in Chapter 5. This chapter focuses on c...