We present WFPC2 images in the F450W, F606W and F814W filters of the interacting pair of galaxies NGC 454. Our data indicate that the system is in the early stages of interaction. A population of young star-clusters has formed around the late component, and substantial amounts of gas have sunk into the center of the earlier component, where it has not yet produced significant visible star forma...

We analysed two sites within and outside an urban development in a rural background to estimate the fungal richness, diversity and community composition in Quercus spp. ectomycorrhizas using massively parallel 454-sequencing in combination with DNA-tagging. Our analyses indicated that shallow sequencing ( approximately 150 sequences) of a large number of samples (192 in total) provided data tha...

Let T be the family of open subsets of a topological space (not necessarily Hausdorff or even T0). We prove that if T has a base of cardinality ≤ μ, λ ≤ μ < 2, λ strong limit of cofinality א0, then T has cardinality ≤ μ or ≥ 2. This is our main conclusion (21). In Theorem 2 we prove it under some set theoretic assumption, which is clear when λ = μ; then we eliminate the assumption by a theorem ...

SUMMARY Massively parallel sequencing technologies hold incredible promise for the study of DNA sequence variation, particularly the identification of variants affecting human disease. The unprecedented throughput and relatively short read lengths of Roche/454, Illumina/Solexa, and other platforms have spurred development of a new generation of sequence alignment algorithms. Yet detection of se...

Genome scans have been used in the studies of ecological speciation to find genomic regions ('outlier loci') showing reduced gene flow between divergent populations/species. High-throughput sequencing ('454') offers new opportunities in this field via transcriptome sequencing. Divergent ecotypes of the marine gastropod Littorina saxatilis represent a good example of incipient ecological speciat...

To increase the yield of DNA sequence generated by the 454 technology from small amounts of starting DNA, we investigated the efficiency of each step in the 454 library preparation process. We find that the last step, when the single-stranded library is released by NaOH, is inefficient and highly variable. When this step is replaced with heat treatment, library amounts dramatically increase. Fu...

UBIQUITIN-PROTEASOME-MEDIATED DEGRADATION, INTRACELLULAR LOCALIZATION, AND PROTEIN SYNTHESIS OF MYOD AND ID1 DURING MUSCLE DIFFERENTIATION Liping Sun‡, Julie S. Trausch-Azar‡, Aaron Ciechanover§ and Alan L. Schwartz‡ From the ‡ Edward Malincrodt Department of Pediatrics and Molecular biology and Pharmacology, Washington University School of Medicine and St.Louis Children’s Hospital, St.Louis, M...

The New Zealand South Hampshire sheep have been well characterised as an animal model for variant late-infantile neuronal ceroid lipofuscinosis (vLINCL) in humans. The disease causing gene has been identified as CLN6, but so far no mutation has been identified. A sheep BAC containing CLN6 and flanking region (~120kb) was sequenced at 13.49-fold sequence coverage using the 454 sequencing method ...