INTRODUCTION Photodynamic therapy (PDT) using 5-aminolevulinic acid-induced protoporphyrin IX (ALA-PpIX) constitutes an interesting alternative for cutaneous leishmaniasis treatment. OBJECTIVE To evaluate the production of PpIXbased on the administration of ALA and MAL and the effect of ALA-PDTat cellular level on non-infected and infected THP-1 cells using Leishmania ( Viannia ) panamensis o...

Plants are frequently subjected to different kinds of stress, such as salinity and, like other organisms, they have evolved strategies for preventing and repairing cellular damage caused by salt stress. Glycine max L. plants were subjected to different NaCl concentrations (0-200 mM) for 10 days. Treatments with 100 and 200 mM NaCl induced ion leakage and lipid peroxidation augmentation, loss in...

Minor malformations (mM) are mild physical deformities that with their incidence, number and evolution may be external indicators of hidden, more serious disorders. Most often these are recognized by the neonatologists. First studies done some forty years ago showed an average incidence of 15% in the general population of newborns and about 50% in children with major malformations (MM). A study...

The GITC/CsCl method (1) is frequently utilized for the isolation of intact RNA. Here we describe the use of this method for the extraction of DNA binding proteins suitable for specific binding site blotting. Cells are tysed by guanidinium isothiocyanate and after the overnight ultracentrifugation step the supernatant of the gradient containing denatured cellular proteins is diarysed against bu...

The present work aims to study the effects of working fluids on thermal performance heat pipe with a wick and in horizontal position. experiments were conducted using copper 20.8 mm inner diameter, length evaporator, condenser, adiabatic regions 300 mm, 350 respectively. selected water, Methanol, ethanol, different binary mixtures (50: 50) %, (30: 70) (70: 30) % mixing ratios. filling ratio was...

The effect of ethanol (EtOH) on synaptic transmission mediated by N-methyl-D-aspartate (NMDA) and non-NMDA glutamate receptors was investigated in slices from adult rat hippocampus. Synaptic responses were elicited by stimulation of stratum radiatum and were recorded in CA1 stratum radiatum or stratum pyramidale. Population EPSPs (pEPSPs) mediated by NMDA receptor activation were isolated by ap...

BENCHMARKS Random amplified polymorphic DNA (RAPD) is widely used with whole cell DNA to determine genetic diversity among plants. The method is also useful when applied specifically to mitochondrial DNA (mtDNA) for plant genotyping and for characterization of organelle-associated changes such as cytoplasmic male sterility (CMS; References 1–3). Some have found that mitochondrial-specific RAPD ...

PURPOSE To evaluate the retention of bone around implants placed immediately following tooth extraction and used to support dental prostheses. MATERIALS AND METHODS Patients from a previous study of implants placed immediately following tooth extraction were recalled to the original practice to obtain dental radiographs, which were then used to compare bone levels after 1 to 22 years of clini...

The effect of various concentrations of CaCl2 and KCl on egg white proteins during isoelectric precipitation of ovomucin was investigated in this study. At low concentrations of CaCl2 (<50 mM), lysozyme was the major contaminant in the precipitated ovomucin, whereas ovalbumin was the predominant one at high concentrations (>or=100 mM). At 50 mM CaCl2 concentration, the concentrations of both ly...