Thus far, no transcription factor IIIA (TFIIIA) from higher plants has been cloned and characterized. We have cloned and characterized TFIIIA and ribosomal protein L5 from Arabidopsis thaliana. Primary sequence comparison revealed a high divergence of AtTFIIIA and a relatively high conservation of AtL5 when compared with other organisms. The AtTFIIIA cDNA encodes a protein with nine Cys(2)-His(...

Detailed karyotypes of 4 lily species (Lilium regale, L. duchartrei, L. brownii var viridium and L. leucanthum var. centifolium), native to Qinling Moutains in China, were constructed on the basis of chromosome arm lengths and fluorescence in situ hybridisation (FISH) with the 45S and 5S rDNA sequences as probes. Results showed that the relative length of these 4 genomes were all around 300μm, ...

With the rapid growth in smartphone technologies and improvement in their navigation sensors, an increasing amount of location information is now available, opening the road to the provision of new Intelligent Transportation System (ITS) services. Current smartphone devices embody miniaturized Global Navigation Satellite System (GNSS), Inertial Measurement Unit (IMU) and other sensors capable o...

To estimate the phylogenetic position of each bacterial species, ACKNOWLEDGEMENTS the nucleotide sequences of the 5S nbosomal RNAs from four Gram-negative marine eubacteria, Vibrio adaptatus, V. This work was supported in part by the following grants; NSF cyclosites, V. hoUisae and V. neocistes were determined by #BSR-8806509, EPA #CR812246 and ONR chemical and enzymatic methods (1). The 5S rRN...

Two photoreactive diazirine derivatives of uridine were used to study contacts between 5S rRNA and 23 rRNA in situ in Escherichia coli ribosomes. 2'-Amino-2'-deoxy-uridine or 5-methyleneaminouridine were introduced into 5S rRNA by T7 transcription. After incorporation of these uridine analogues into the transcript their amino groups were modified with 4-[3-(trifluoromethyl)-3 H -diazirin-3-yl]b...

Stably transfected cell lines expressing the wild-type rat LH/CG receptor (rLHR) or a full-length rLHR in which S635, T638, S639, S649 and S653 were simultaneously mutated to alanine residues (designated rLHR-5S/T-->A) were used to probe the importance of receptor phosphorylation on the regulation of receptor functions. The mutant receptor binds hCG with high affinity and transduces the hormona...

The addition of 4 eq of chloral to osmundalactone (4S,5R)-4 gave quantitative formation of the hemiacetal derivative (4S,5R)-8, which was treated with methane sulfonic acid to afford the intramolecular Micheal addition product (+)-(3S,4S,5R)-9 possessing a 3,4-cis-dihydroxy-δ-lactone in 78% overall yield from (4S,5R)-4. The obtained (+)-(3S,4S,5R)-9 was subsequently converted to methyl D-digito...

Allopolyploidy is a significant evolutionary process, resulting in new species with diploid or greater chromosome complements derived from two or more progenitor species. We examined the chromosomal consequences of genomic merger in Arabidopsis suecica, the allotetraploid hybrid of Arabidopsis thaliana and Arabidopsis arenosa. Fluorescence in situ hybridization with centromere, nucleolus organi...

Two additional new compounds, pseudellone D (1) and (5S,6S)-dihydroxylasiodiplodin (3), along with the two known compounds lasiodipline F (2), (5S)-hydroxylasiodiplodin (4) were isolated from the marine-derived fungus Pseudallescheria ellipsoidea F42-3 associated with the soft coral Lobophytum crassum. Their structures, including absolute configurations, were elucidated on the basis of the corr...