Friable calli of Polygonum multiflorum Thunb have been induced in MS medium supplemented with 6-benzylaminopurine (6-BA) and kinetin (KT). Suspension cultures were initiated from friable calli by inoculating calli in liquid MS medium in shake flasks in the dark and 25 °C on an orbital shaker at 100 rpm. The maximum dry weight (DW, 7.85 g/L) and 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glycoside (T...

In vitro organogenesis may provide a method to reduce the very large number of observations required for in planta studies on seed set in Fagopyrum esculentum. Plants were regenerated from leaf petiole explant tissue of buckwheat seedlings. Maximum shoot regeneration was induced on Murashige and Skoog (MS) medium containing Gamborg’s vitamins, 6-benzylaminopurine (BA), 6, -dimethylallylamino-pu...

A procedure for rapid clonal propagation of Passiflora edulis Sims. f. flavicarpa Deg. (Passifloraceae) has been developed in this study. Nodal explants were sterilized with 0.1% HgCl2 and inoculated on Murashige and Skoog (MS) basal medium. The addition of 2.0 mgL(-1) 6-benzylaminopurine (BAP) to MS medium caused an extensive proliferation of multiple shoots (8.21 ± 1.13) primordial from the n...

The tomato (Solanum lycopersicum L.) is a major crop of global economic significance. characterization genes associated with agriculturally important traits often performed using genetic transformation. To achieve an efficient transformation protocol, three components are required, namely, regenerable target tissue, DNA delivery method, and robust transformant selection system. present study wa...

The objective of this study was to establish an efficient—direct or indirect—regeneration system for pineapple (Ananas comosus L.) plants, with a high rate multiplication and that would preserve the genetic identity donor genotype (Hybrid ‘MD2’) in regenerated plants. Ten treatments, different concentrations 2,4-Dichlorophenoxyacetic (2,4-D) Picloram (P), absence presence 6-Benzylaminopurine (B...

This study aims to investigate whether the in vitro-cultured L. pumila var. alata has higher antioxidant activity than its wild plant. An 8-week-old nodal segment and leaf explants were cultured onto Murashige Skoog (MS) medium supplemented with various cytokinins (zeatin, kinetin, 6-benzylaminopurine (BAP)) for shoot multiplication auxins (2,4-dichlorophenoxyacetic acid (2,4-D) picloram) callu...

Campanula phitosiana Yıldırım & Şentürk, is a local endemic to the Aydın Mountain range, which distribute across in Western Anatolia (both İzmir and Aydın). This species belongs Mediterranean basin floristic region. According International Union for Conservation of Nature’s criteria, C. classified as “Critically Endangered” (CR). The aim study develop vitro regeneration protocol critica...

Abstract Hairy root cultures are an efficient tool for the biotechnological production of plant metabolites and a convenient experimental model analyzing effect various compounds on metabolism. In contrast to many other types in vitro cultures, hairy roots do not require external supply phytohormones medium. Consequently, growth regulators such as auxins cytokinins rarely used elicitors culture...