In order to investigate the crucial role of ZmBT1 in starch accumulation during maize grain development and analyze expression distribution various tissues, we prepared a polyclonal antibody. Specifically, successfully expressed recombinant plasmid pGEX-6p-ZmBT1-C (382-437aa) purified Gst-ZmBT1-C as antigen for antibody preparation. Our results confirmed that protein tissues can be specifically...

Several papers have recently shown that 6–7% of retarded patients with unclassified malformation syndromes and normal routine cytogenetic analysis have cryptic rearrangements involving subtelomeric regions. About half of these patients have familial unbalanced translocations, the other half have de novo deletions, and very few cases have duplications. The existence of subtelomeric imbalances wi...

During activation of the spliceosome, the U4/U6 snRNA duplex is dissociated, releasing U6 for subsequent base pairing with U2 snRNA. Proteins that directly bind the U4/U6 interaction domain potentially could mediate these structural changes. We thus investigated binding of the human U4/U6-specific proteins, 15.5K, 61K and the 20/60/90K protein complex, to U4/U6 snRNA in vitro. We demonstrate th...

the aerial parts of salvia glutinosa afforded in addition to lupeol, a - and ? - amyrin a furanosesquiterpene, its structure being established by high field nmr techniques. the relative configuration at c-1, c-10, c-4, c-5 and c-6 of 6?tigloyloxyglechomafuran 1 was determined by the observed noe's

Immunogenetic evidence suggests a genetic association between the major histocompatibility complex and the two genitourinary neoplasms, testicular teratocarcinoma and renal cell carcinoma. In order to develop a possible explanation for these findings, we designed a series of experiments to investigate the existence of a tumor suppressor gene in the region of HLA by looking for loss of germ line...

BACKGROUND Reading disabilities (RD) and attention-deficit hyperactivity/disorder (ADHD) are two common childhood disorders that co-occur by chance more often than expected. Twin studies and overlapping genetic linkage findings indicate that shared genetic factors partially contribute to this comorbidity. Linkage of ADHD to 6p, an identified RD candidate locus, has previously been reported, sug...