OBJECTIVE To verify the phenotype to genotype correlations of mitochondrial DNA (mtDNA) related disorders in an atypical maternally inherited encephalomyopathy. METHODS Neuroradiological, morphological, biochemical, and molecular genetic analyses were performed on the affected members of a pedigree harbouring the heteroplasmic A to G transition at nucleotide 3243 of the mitochondrial tRNALeu(...

Received 31 October 1995 and in final revised form 19 February 1996 Accepted 23 February 1996 Abstract Objective-To verify the phenotype to genotype correlations of mitochondrial DNA (mtDNA) related disorders in an atypical maternally inherited encephalomyopathy. Methods-Neuroradiological, morphological, biochemical, and molecular genetic analyses were performed on the affected members of a ped...

It has been reported that human mesenchymal stem cells (MSCs) can transfer mitochondria to the cells with severely compromised mitochondrial function. We tested whether the reported intercellular mitochondrial transfer could be replicated in different types of cells or under different experimental conditions, and tried to elucidate possible mechanism. Using biochemical selection methods, we fou...

OBJECTIVE Young adults with newly diagnosed apparent type 2 diabetes present the clinician with a wide differential diagnosis of possible etiology, including autoimmune and genetic causes as well as young-onset type 2 diabetes (YT2D). The characteristics of these groups have been described, but it is not known in which subjects investigation for etiology may be beneficial. RESEARCH DESIGN AND...

PURPOSE To investigate retinal function in patients with maternally inherited diabetes and deafness (MIDD) and to correlate the findings with fundus autofluorescence (FAF) imaging. METHODS FAF was imaged in five patients (age range, 49-60 years) confirmed to have the mitochondrial DNA nucleotide A3243G point mutation. Retinal function was measured by full-field (Ganzfeld) electroretinography ...

1) Introduction 2) What is a microarray? a) Experimental steps in a typical cDNA microarray experiment b) If you don?t see it, it doesn?t mean that it isn?t there. 3) Experimental Design a) Comparing independent ?replicate? arrays b) ?A vs. M? plot. 4) Informatic Annotation of Array Data 5) Initial annotation of gene list based on published literature a) Linking array data to the literature b) ...

The problem of low space utilization (Fig. 1). E mbedded/mobile computing systems are usually battery-powered devices and are widely adopted in various application domains. Due to cost and energy considerations, they usually have limited computing power, RAM space, and storage capacity. However, recent improvements in embedded/ mobile computing systems' computing ability have allowed some embed...